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SCT277 BioTracker™ Red pH-Independent Autophagy Live Cell Probe

Fluorescence microscopy
Flow cytometry

SCT277
5 nmol  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberSCT277
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DescriptionBioTracker™ Red pH-Independent Autophagy Live Cell Probe
OverviewThe small fluorescent molecule SCT277 is used to detect autophagosomes and autolysosomes. The presumed mechanism is that the dye is incorporated into the autophagosome during double-membrane formation via structural features, and then emits fluorescence under hydrophobic conditions. The probe emits in the far red range.

SPECTRAL CHARACTERISTICS:
Excitation λ: 530 nm
Emission λ: 720 nm
Alternate Names
  • red autophagy probe
  • autophagosome live cell probe
  • red autophagosome probe
  • red AP live cell probe
Background InformationBioTracker Red pH-Independent Autophagy Live Cell Probe detects autophagosomes and autolysosomes. The presumptive mechanism involves incorporation of the probe via structural features into the autophagosome during double-membrane formation. Upon incorporation, the probe emits fluorescence under hydrophobic conditions. The utility of the probe is conferred by molecular properties that include cell permeability, does not require transfection, and enables live cell detection with fluorescence microscopy or other fluorescence capture instruments.
Product Information
Detection methodFlow cytometry Fluorescence
FormatLyophilized solid
PresentationLyophilized solid.
Quality LevelMQ100
Applications
ApplicationFluorescence microscopy
Flow cytometry
Key Applications
  • Cell Based Assays
  • Fluorescence
  • Flow Cytometry
  • Fluorescence Activated Cell Sorting (FACS)
Application NotesSCT277 detects autophagosomes and autolysosomes by the likely mechanism of incorporation into the autophagosome during double membrane formation, and emits red fluorescence under hydrophobic conditions. SCT277 is cell permeable, does not require transfection, and enables live cell imaging with fluorescence microscopy and quantitative assay by flow cytometry.

Add 50 uL DMSO to tube of SCT277 (5 nmol) and pipet to dissolve to make a 0.1 mM stock solution. Dilute with culture medium to 0.1 - 0.5 uM to make working solution.
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at 2 - 8 °C, protected from light.
Packaging Information
Material Size5 nmol
Global Trade Item Number
Catalogue Number GTIN
SCT277 04065269218579