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72351 BacMagic™-3 Transfection Kit - Novagen

BacMagic™-3 Transfection Kit - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
72351
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
72351-3 5 rxn
Description
OverviewThe BacMagic™-3 Transfection Kit provides a positive selection for creating baculovirus recombinants in preparation for recombinant protein expression in insect cells. The kit improves on the traditional method for generating recombinant baculoviruses by eliminating the tedious, time-consuming steps of plaque purification. Derived from an AcNPV genome, BacMagic-3 DNA is deleted for a portion of the essential open reading frame (ORF) 1629, preventing nonrecombinant virus form replicating in insect cells. The nonessential chitinase (chiA) gene is deleted to improve production of secreted and membrane target proteins. Additionally, the v-cath gene, a cathespsin-like cysteine protease, is deleted resulting in significantly improved quality and yield for most target proteins. Unlike BacMagic™-2, the BacMagic-3 genome is deleted for an additional three viral genes (p10, p24, and p26). These genes are nonessential to virus in tissue culture and improve protein expression. The BacMagic-3 Transfection Kit contains the BacMagic-3 DNA, Insect GeneJuice® Transfection Reagent, and a positive control plasmid to monitor co-transfection efficiency. For optimal performance, Sf9 cells and BacVector® Medium are also included in the transfection kit.
Not available for sale in Japan
Catalogue Number72351
Brand Family Novagen®
Application Data
Figure 1. Construction of baculovirus recombinants with three BacMagic systems

Figure 2. Streamlined baculovirus method
References
Product Information
•0.5 µgBacMagic-3 DNA
•50 µlInsect GeneJuice Transfection Reagent
•2 x 1 mlSf9 Cells
•1 LBacVector® Insect Cell Medium
•2 µgTransfection Control Plasmid
DeclarationNot available for sale in Japan
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Multiple Storage Conditions
Toxicity Regulatory Review
Storage Multiple storage requirements
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
72351-3 04055977266566

Documentation

BacMagic™-3 Transfection Kit - Novagen Certificates of Analysis

TitleLot Number
72351

User Protocols

Title
TB459 BacMagic™ DNA Kits