Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

69451 BL21(DE3)pLysS Competent Cells - Novagen

BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.

BL21(DE3)pLysS Competent Cells - Novagen MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
69451
Purchase on Sigma-Aldrich

Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
69451-3 Glass bottle 0.4 ml
69451-4 Glass bottle 1 ml
Description
OverviewBL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability.
Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR)


This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number69451
Brand Family Novagen®
References
Product Information
Components
Guaranteed efficiency> 2 x 10⁷ cfu/μg
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
69451-3 04055977274356
69451-4 04055977274363

Documentation

BL21(DE3)pLysS Competent Cells - Novagen SDS

Title

Safety Data Sheet (SDS) 

BL21(DE3)pLysS Competent Cells - Novagen Certificates of Analysis

TitleLot Number
69451

Citations

Title
  • Alvarez, V.E., et al. 2008. Journal of Biological Chemistry 283, 3454.
  • Khan, M.A., et al. 2008. Journal of Biological Chemistry 283, 1340.
  • Luttge, B.G., et al. 2008. Journal of Virology 82, 2106.
  • Mittra, B., et al. 2008. Journal of Biological Chemistry 283, 3161.
  • Geisler, D.A., et al. 2007. Journal of Biological Chemistry 282, 28455.
  • Wu, A.T.H., et al. 2007. Journal of Biological Chemistry 282, 12164.
  • Yu, Z., et al. 2007. Nucleic Acids Research 35, 2107.
  • Gong, B., et al. 2006. Cell 126, 775.
  • Annika Grabbe and Jurgen Wienands. 2006. Blood 108, 3761.
  • Grobe, A.C., et al. 2006. American Journal of Physiology: Lung 290, L1069.
  • LeFebvre, A.K., et al. 2006. Journal of Biological Chemistry 281, 22917.
  • Schmelz, E.A., et al. 2006. Proceedings of the National Academy of Sciences (USA) 103, 8894.
  • Ellen J. Tisdale and Cristina R. Artalejo. 2006. Journal of Biological Chemistry 281, 8436.
  • M. Wang and K. A. Hudak. 2006. Nucleic Acids Research 34, 1174.
  • Ashida, H., et al. 2005. Plant and Cell Physiology 46, 557.
  • Attinger, A., et al. 2005. Journal of Immunology 174, 3501.
  • Banci, L., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 3994.
  • Hsiu-Hui Chen, H-H., et al. 2005. Blood 105, 3902.
  • Cherny, I.,et al. 2005. Journal of Biological Chemistry 280, 30063.
  • Chiarelli, L.R., et al. 2005. Blood 105, 3340.
  • Dattelbaum, J.D., et al. 2005. Protein Science 14, 284.
  • Dufour, E.K., et al. 2005. Protein Science 14, 303.
  • Flint, J., et al. 2005. Journal of Biological Chemistry 280, 23718.
  • Funa, N., et al. 2005. Journal of Biological Chemistry 280, 14514.
  • Hamburger, A.E., et al. 2005. Journal of Biological Chemistry 280, 12567.
  • Handa, N., et al. 2005. Protein Science 14, 1004.
  • Herron, B.J., et al. 2005. Human Molecular Genetics 14, 667.
  • Islam, M.N., et al. 2005. Protein Engineering Design and Selection 18, 71.
  • Kawakami, H., et al. 2005. Journal of Biological Chemistry 280, 27420.
  • Han Ie Kim and Seung-Taek Lee. 2005. Journal of Biological Chemistry 280, 28973.
  • Kim, S.H., et al. 2005. Molecular and Cellular Proteomics 4, 267.
  • Korneeva, N.L., et al. 2005. Journal of Biological Chemistry 280, 1872.
  • Kotoshiba, S., et al. 2005. Journal of Biological Chemistry 280, 17694.
  • Kwade, Z., et al. 2005. Journal of Biological Chemistry 280, 17512.
  • Li, C., et al. 2005. Journal of Biological Chemistry 280, 24864.
  • Li, H., et al. 2005. Protein Science 14, 3025.
  • Madsen, K.L., et al. 2005. Journal of Biological Chemistry 280, 20539.
  • Mori, S., et al. 2005. Journal of Biological Chemistry 280, 24104.
  • Mukhopadhyay, S., et al. 2005. Journal of Biological Chemistry 280, 538.
  • Nowotny, M., et al. 2005. Cell 121, 1005.
  • Ruthenburg, A.J., et al. 2005. Journal of Biological Chemistry 280, 26177.
  • Suzuki, T., et al. 2005. Plant and Cell Physiology 46, 1452.
  • Tóth, K.F., et al. 2005 Journal of Biological Chemistry 280, 15690.
  • Takahashi, H., et al. 2005. Journal of Biological Chemistry 280, 5611.
  • Tang, Q-Q., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 9766.
  • Yamauchi, J., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 5198.
  • Bühling, F., et al. 2004. Clin. Diagn. Lab. Immunol. 11, 131.
  • Bittker, J.A., et al. 2004. Procedings of the National Academy of Science 101, 7011.
  • Yang Chao and Dax Fu. 2004. Journal of Biological Chemistry 279, 17173.
  • Tran C. Dong and Simon M. Cutting. 2004. Journal of Biological Chemistry 279, 43468.
  • Ferreira, V., et al. 2004. The Journal of Immunology 172, 3042.
  • Gu, D., et al. 2004. Journal of Biological Chemistry 279, 31171.
  • Jeong, S.Y., et al. 2004. Planta 219, 298.
  • Richard, C.L., et al. 2004. Journal of Bacteriology 186, 740.
  • Sanfelice, D., et al. 2004. Journal of Biomolecular NMR 29, 449.
  • Tisdale, E.J., et al. 2004. Journal of Biological Chemistry 279, 54046.
  • Xiao, K., et al. 2004. Journal of Biological Chemistry 279, 55744.
  • Zhang, Y.X., et al. 2004. Cell Research 14, 208.
  • Albers, M., et al. 2003. RNA 9, 138.
  • BMazumder, B., et al. 2003. Cell 115, 187.
  • Premkumar, L., et al. 2003. Protein Expression and Purification 28, 151.
  • Huhtinen, K., et al. 2002. Journal of Biological Chemistry 277, 3424.
  • Renshaw, P.R., et al. 2002. Journal of Biological Chemistry 277, 21598.
  • Chazalet, V., et al. 2001. Journal of Bacteriology 183, 7067.
  • Glick, E., et al. 2001. European Molecular Biology Organization Journal 20, 7303.
  • Brandt, J.J., et al. 2000. Protein Expression and Purification 20, 300.
  • Cabral, J.H.M., et al. 1998. Cell 95, 649.
  • A.K. Gombert and B.V. Kilikan. 1998. Journal of Biotechnology 60, 47.
  • Vinkemeir, U., et al. 1998. Science 279, 1048.
  • Black, S.M., et al. 1997. Journal of Clinical Investigation 100, 1448.
  • Bochkarev, A., et al. 1997. Nature 385, 176.
  • Shanley, T.P., et al. 1997.

    • User Protocols

    Title
    TB009 Competent Cells
    TB053 Academic and Non-profit Laboratory Assurance Letter