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ALP30 Apolipoprotein B, human

Apolipoprotein B, human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Positive Control UniProt Number: P04114 Entrez Gene Number: NM_000384.2
ALP30
500 µg  
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Overview

Replacement Information
Description
Catalogue NumberALP30
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionApolipoprotein B, human
OverviewTwo forms of Apo B exist: Apo B-100 and Apo B-48. The first is found in VLDL and LDL and is produced by the liver. The second is found in chylomicrons and originates in the intestine.

Apo B is purified from LDL isolated by density gradient ultracentrifugation (1.03-1.05 g/mL density range) followed by size exclusion chromatography in 10 mM deoxycholate, 50 mM NaCl, 50 mM Na2CO3, pH 10 (Walsh & Atkinson 1983).

PROPERTIES:

Apo B, which has a Mr of 550 kD, is the major protein found in LDL and is responsible for the cellular uptake of LDL particles from the plasma (Goldstein & Brown 1982). The Apo B prepared by this procedure is aggregated. The aggregates are not retained by a 0.45 mm filter, but migrate only a short distance into a 5% SDS PAGE gel. In the isolation buffer, Apo B gives a stable solution that is slightly hazy.
Alternate Names
  • ApoB
References
Product Information
PresentationLyophilized from 50 mM Na2CO3, pH 10, 50 mM NaCl, and 10 mM sodium deoxycholate solution. Non-sterile.
Quality LevelMQ100
Applications
Key Applications
  • Positive Control
Biological Information
Purity>95% by SDS-PAGE; Single arc by IEP against antisera to whole human serum.
SourceHuman plasma, fresh, non-frozen. Tested negative for anti-HIV-1, anti-HIV-2, anti-HCV, anti-HBc and HBsAg.
Entrez Gene Number
Entrez Gene SummaryThis gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination.
Gene Symbol
  • APOB
  • apoB-48
  • FLDB
  • apoB-100
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P04114 # Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
SIZE: 4563 amino acids; 515563 Da
SUBCELLULAR LOCATION: Secreted.
PTM: Palmitoylated; structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle.
DISEASE: SwissProt: P04114 # Defects in APOB are a cause of familial hypobetalipoproteinemia (FHBL) [MIM:107730]. FHBL is a genetically heterogeneous autosomal co-dominant disorder, associated with reduced plasma concentrations of apoB, LDL and VLDL. Heterozygotes for FHBL are usually asymptomatic with LDL cholesterol and apoB- 100 concentrations less than 50% of those in normal plasma. Homozygotes have extremely low plasma LDL cholesterol and apoB-100 concentrations, and clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia [MIM:200100]. & Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB) [MIM:144010]. FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. & Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.SIMILARITY:SwissProt: P04114 ## Contains 1 vitellogenin domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at <= -20°C for up to six months. Reconstitute to 1 mL by adding deionized water. If the deoxycholate is removed, Apo B-100 will slowly aggregate and become insoluble. After reconstitution maintain at +2-8°C for up to 4 weeks.
Packaging Information
Material Size500 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ALP30 04053252474651

Documentation

Apolipoprotein B, human SDS

Title

Safety Data Sheet (SDS) 

Apolipoprotein B, human Certificates of Analysis

TitleLot Number
HUMAN APOLIPOPROTEIN B 3140035
HUMAN APOLIPOPROTEIN B 2900875
HUMAN APOLIPOPROTEIN B 2896418
HUMAN APOLIPOPROTEIN B 3106557
HUMAN APOLIPOPROTEIN B 3584422
HUMAN APOLIPOPROTEIN B - 2435083 2435083
HUMAN APOLIPOPROTEIN B - 2246756 2246756
HUMAN APOLIPOPROTEIN B - 3030273 3030273
HUMAN APOLIPOPROTEIN B - 3061235 3061235
HUMAN APOLIPOPROTEIN B - 3188658 3188658
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