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MABN824 Anti-α-syn Antibody (amino acids 115-122), clone LB509

MABN824
100 µg  
Purchase on Sigma-Aldrich

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Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN824
DescriptionAnti-α-syn Antibody (amino acids 115-122), clone LB509
Alternate Names
  • Alpha-synuclein
  • Non-A beta component of AD amyloid
  • Non-A4 component of amyloid precursor
  • NACP
Background Informationalpha-Synuclein is a small (140 amino acid) cytoplasmic protein of unclear function that is enriched in presynaptic terminals and is the precursor protein of a nonamyloid beta component of senile plaques in Alzeheimer's Disease (AD). α-Synuclein is located predominantly in the presynaptic nerve terminals in the brain but is also found in low concentrations in all tissues except liver. Studies have shown that abnormal aggregation and accumulation of synaptic proteins, including α-Synuclein, might be associated with plaque formation in AD and a major component of Lewy bodies in dementia with Lewy bodies (DLB). It has also been identified in the Glial Cytoplasmic Inclusions (GCIs) found in Multiple System Atrophy (MSA). Clone LB509 detects epitope in the region from amino acid 115 to 122 and has been used to characterize the digestion patterns of different forms of in vitro prepared alpha-synclulein aggregates (Guo, J.L., et al. 2013; PMID 23827677).
References
Product Information
FormatPurified
HS Code3002 15 90
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-α-syn Antibody (amino acids 115-122), clone LB509 is an antibody against α-syn for use in Western Blotting and Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected α-syn in human cerebral cortex tissue.
Western Blotting Analysis: A representative lot detected α-syn in PK-digested FL a-syn pffs (Guo, J.L., et al. (2013). Cell. 154:103-117).
Biological Information
ImmunogenConjugated linear peptide corresponding to human α-syn (aa 115-122).
CloneLB509
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityThis antibody detects a-Synuclein preformed fibrils that can cross seed tau fibrilliation.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • SNCA
  • NACP
  • PARK1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~15 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected α-syn in 10 µg of human brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN824 04055977144970

Documentation

Anti-α-syn Antibody (amino acids 115-122), clone LB509 Certificates of Analysis

TitleLot Number
Anti-a-Synuclein, (aa 115-122) -2822511 2822511
Anti-a-Synuclein, (aa 115-122) -Q2559146 Q2559146
Anti-a-Synuclein, (aa 115-122) clone LB509 - 2986025 2986025
Anti-a-Synuclein, (aa 115-122) clone LB509 - 3195403 3195403
Anti-a-Synuclein, (aa 115-122) clone LB509 - 3381379 3381379
Anti-a-Synuclein, (aa 115-122) clone LB509 - 3728238 3728238
Anti-a-Synuclein, (aa 115-122) clone LB509 - 3850519 3850519
Anti-a-Synuclein, (aa 115-122) clone LB509 - 3929932 3929932
Anti-a-Synuclein, (aa 115-122) clone LB509 - 3984658 3984658
Anti-a-Synuclein, (aa 115-122) clone LB509 - 4069734 4069734

References

Reference overviewPub Med ID
Distinct α-synuclein strains differentially promote tau inclusions in neurons.
Guo, JL; Covell, DJ; Daniels, JP; Iba, M; Stieber, A; Zhang, B; Riddle, DM; Kwong, LK; Xu, Y; Trojanowski, JQ; Lee, VM
Cell  154  103-17  2013

Show Abstract
23827677 23827677

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Life Science Research > Antibodies and Assays > Primary Antibodies