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MABE350 Anti-phospho-RNAPII (Tyr1) Antibody, clone 3D12

Detect the phospho-RNAPII (Tyr1) protein using this Anti-phospho-RNAPII (Tyr1), clone 3D12 validated for use in WB, IP & ChIP.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: POLR2A, POLR2 UniProt Number: P24928
MABE350
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IP, ChIPRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE350
DescriptionAnti-phospho-RNAPII (Tyr1) Antibody, clone 3D12
Alternate Names
  • DNA-directed RNA polymerase II subunit RPB1
  • RNA polymerase II subunit B1
  • DNA-directed RNA polymerase II subunit A
  • DNA-directed RNA polymerase III largest subunit
  • RNA-directed RNA polymerase II subunit RPB1
Background InformationRNA polymerase II (RNAPII or Pol II) is a multi-subunit enzyme responsible for the transcription of transcription of DNA into RNA from protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. RNAPII can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
Product Information
FormatPurified
Control
  • Untreated and lambda phosphatase treated HeLa cell lysates
PresentationPurified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect the phospho-RNAPII (Tyr1) protein using this Anti-phospho-RNAPII (Tyr1), clone 3D12 validated for use in WB, IP & ChIP.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Chromatin Immunoprecipitation (ChIP)
Application NotesImmunoprecipitation Analysis: A representative lot from an independent laboratory detected phospho-RNAPII (Tyr1) in proliferating yeast whole cell extract (Mayer, A., et al. (2012). Science. 336(6089):1723-1725.).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot from an independent laboratory immunoprecipitated phospho-RNAPII (Tyr1) in proliferating yeast whole cell extract (Mayer, A., et al. (2012). Science. 336(6089):1723-1725.).
Biological Information
ImmunogenOvalbumin-conjugated linear peptide corresponding to human RNAPII phosphorylated at Tyr1.
Clone3D12
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • POLR2A
  • POLR2
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~220 kDa observed
Product Usage Statements
Quality AssuranceEvaluated by Western Blot Analysis in untreated and lambda phosphatase treated HeLa cell lysates.

Western Blot Analysis: 2 µg/mL of this antibody detected phospho-RNAPII (Tyr1) in 10 µg of untreated HeLa cell lysate. (Lambda phosphatase treatment was performed during the western blot procedure.)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
MABE350 04053252833007