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07-1348 Anti-phospho-PLK1 (Ser137) Antibody

Detect phospho-PLK1 (Ser137) using this Anti-phospho-PLK1 (Ser137) Antibody validated for use in WB.

Anti-phospho-PLK1 (Ser137) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Kinases & Phosphatases Gene Symbol: EC 2.7.11.21, PLK , PLK-1, STPK13 UniProt Number: P53350
07-1348
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, R, BWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1348
DescriptionAnti-phospho-PLK1 (Ser137) Antibody
Alternate Names
  • Polo-like kinase 1
  • Serine/threonine-protein kinase 13
  • cell cycle regulated protein kinase
  • polo (Drosophia)-like kinase
  • polo like kinase
  • polo-like kinase (Drosophila)
  • polo-like kinase 1 (Drosophila)
Background InformationPolo-like Kinase 1 (PLK1) is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Expressed PLK protein accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
Product Information
FormatAffinity Purified
Control
  • Lambda phosphatase treated and untreated human placenta tissue lysate.
PresentationAffinity purified rabbit serum in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect phospho-PLK1 (Ser137) using this Anti-phospho-PLK1 (Ser137) Antibody validated for use in WB.
Key Applications
  • Western Blotting
Application NotesWestern Blot (SNAP ID) Analysis: 0.5 µg/mL of this antibody detected PLK1 phosphorylated at Ser137 in human placenta tissue lysate.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human phosphoylated PLK1 at and around Ser137.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody recognizes phosphorylated PLK1 at Ser137.
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Bovine
Species Reactivity NoteHuman, Rat and Mouse. Predicted to react with Cow based on sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • EC 2.7.11.21
  • PLK
  • PLK-1
  • STPK13
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION: Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
ENZYME REGULATION: Activated by serine and threonine phosphorylation.
SUBUNIT STRUCTURE: Interacts with CEP170 and EVI5. Interacts and phosphorylates ERCC6L. Interacts with FAM29A. Interacts with BTBD12/SLX4. Ref.11 Ref.12 Ref.13 Ref.14 Ref.18
SUBCELLULAR LOCATION:Nucleus.
TISSUE SPECIFICITY:Placenta and colon.
DEVELOPMENTAL STAGE:Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.
INDUCTION: By growth-stimulating agents.
PTM: Catalytic activity is enhanced by phosphorylation of Thr-210 and/or Ser-137.Autophosphorylation and phosphorylation of Ser-137 are not significant events during activation of PLK1 in M phase.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain.
Molecular Weight68 kDa
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in human placenta tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PLK1 phosphorylated at Ser137 in lambda phosphatase treated and untreated human placenta tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8°C for up to 12 months.
Packaging Information
Material Size100 µg
Global Trade Item Number
Catalogue Number GTIN
07-1348 04053252407567