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MABS2269-25UG Anti-phospho-PD-1 (Tyr248) Antibody, clone 407.6G12

Anti-phospho-PD-1 (Tyr248), clone 407.6G12, Cat. No. MABS2269, is a mouse monoclonal antibody that detects PD-1 (CD279) and is tested for use in Western Blotting and Flow Cytometry.

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25 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABS2269-25UG
DescriptionAnti-phospho-PD-1 (Tyr248) Antibody, clone 407.6G12
Alternate Names
  • Programmed cell death protein 1
  • Protein PD-1
  • hPD-1
  • CD279
Background InformationProgrammed cell death protein 1 (UniProt: Q15116; also known as Protein PD-1, hPD-1, CD279) is encoded by the PDCD1 (also known as PD1) gene (Gene ID: 5133) in human. PD-1 is a monomeric inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. PD-1 and its ligands, PD-L1 and PD-L2 play a key role in the maintenance of peripheral tolerance, a process by which the quiescence of autoreactive mature T cells is maintained. PD-1 is a single-pass type I membrane protein, synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off. The mature form contains an extracellular domain (aa 24-170), a transmembrane domain (aa 171-191), and a cytoplasmic domain (aa 192-288). PD-1 also contains a single N-terminal immunoglobulin variable region (IgV) like domain, an ITIM motif (aa 221-226) and an ITSM motif ( aa 247-251). Tyrosine 248 falls in ITSM motif and located within the intracellular domain of PD-1 and, phosphorylation of PD-1 at Tyrosine 248 is a significant post-translational modification that influences its function. Src family kinases, such as Lck and Fyn, are involved in the initial phosphorylation of PD-1. Upon binding of PD-1 to its ligands (PD-L1 or PD-L2), phosphorylation at Tyr248 triggers downstream signaling pathways. PD-1 phosphorylation at Tyr248 promotes the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates de-phosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKC theta and CD247/CD3zeta. The phosphorylation at Tyr248 is crucial for the full inhibitory effect of PD-1 on T-cell activity. It contributes to the suppression of T-cell proliferation, cytokine production, and cytotoxic activity. Studies demonstrate that phosphorylation of the ITSM motif of PD-1 marks dysfunctional T cells that may be rescued with PD-1 blockade. Detection of phospho-PD-1 in TILs is a potential biomarker for PD-1 immunotherapy responses. (Ref.: Bu, X., et al. (2021). Cancer Immunol Res. 9(12):1465-1475).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-phospho-PD-1 (Tyr248), clone 407.6G12, Cat. No. MABS2269, is a mouse monoclonal antibody that detects PD-1 (CD279) and is tested for use in Western Blotting and Flow Cytometry.
Key Applications
  • Western Blotting
  • Flow Cytometry
Application NotesTested Applications

Western Blotting Analysis: A representative lot detected PD-1 (CD279) in Western Blotting application (Bu, X., et al. (2021). Cancer Immunol Res. 9(12):1465-1475).

Flow Cytometry Analysis: A representative lot detected PD-1 (CD279) in Flow Cytometry application (Bu, X., et al. (2021). Cancer Immunol Res. 9(12):1465-1475).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids surrounding phospho-tyrosine 248 from the C-terminal half of human PD-1 (CD279).
EpitopeC-terminal half
Clone407.6G12
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 407.6G12 is a mouse monoclonal antibody that specifically detects PD-1 (CD279) phosphorylated on tyrosine 248.
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with Monkey based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PDCD1
Modifications
  • Phosphorylation
Purification MethodProtein G purified
UniProt Number
Molecular WeightTarget molecular weight ~55 kDa observed; 31.65 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from Jurkat cells treated with pervanadate (0.1 mM; 5 min.at 37C).

Western Blotting Analysis (WB): A 1:1000 dilution of this antibody detected PD-1 phosphorylated on tyrosine 248 in lysate from Jurkat cells treated with pervanadate (0.1 mM; 5 min. at 37C), but not in lysate from untreated Jurkat cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS2269-25UG 04065272163569