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05-636-AF488 Anti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 488 Conjugate Antibody

This Anti-phospho Histone H2A.X (Ser139) antibody, clone JBW301, Alexa Fluor® 488 Conjugate is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epitope Tags & General Use Research Sub-Category: Epitope Tags Gene Symbol: H2AFX, H2AX UniProt Number: P16104
05-636-AF488
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue Number05-636-AF488
DescriptionAnti-phospho Histone H2A.X (Ser139), clone JBW301, Alexa Fluor® 488 Conjugate Antibody
Alternate Names
  • Histone H2AX
  • H2a/x
  • Histone H2A.X
Background InformationHistone H2A.X is a variant of histone H2A, and is similarly associated with genomic DNA. However, histone is structurally different from other members of the H2A family in possessing a C-terminal tail that contains the Ser139 residue that is phosphorylated in response to breaks in double-stranded DNA. The phosphorylation of H2A.X is a rapid process that is mediated by ATM/ATR proteins.
Product Information
FormatAlexaFluor®488
PresentationPurified mouse conjugate in buffer containing PBS, 15 mg/mL BSA, and 0.1% Sodium Azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-phospho Histone H2A.X (Ser139) antibody, clone JBW301, Alexa Fluor® 488 Conjugate is validated for use in ICC for the detection of phospho Histone H2A.X (Ser139).
Key Applications
  • Immunocytochemistry
Biological Information
ImmunogenLinear peptide corresponding to phospho-Histone H2A.X (Ser139).
CloneJBW301
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Vertebrates based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • H2AFX
  • H2AX
Purification MethodProtein G Purified
UniProt Number
Molecular Weight17 kDa calculated
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in untreated (negative control) and Staurosporin treated HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Histone H2A.X (Ser139) in Staurosporin treated HeLa cells, and not in untreated HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
05-636-AF488 04053252971907