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07-745-I Anti-phospho Histone H2A (Ser129) Antibody

Detect Histone H2A using this rabbit polyclonal antibody, Anti-phospho Histone H2A (Ser129) Antibody validated for use in western blotting, Dot Blot, Peptide Inhibition Assay, IP & ICC.

Anti-phospho Histone H2A (Ser129) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HTA1, H2A1, SPT11 UniProt Number: P04911
07-745-I
100 µg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
YeastWB, DB, PIA, IP, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-745-I
DescriptionAnti-phospho Histone H2A (Ser129) Antibody
Alternate Names
  • Histone H2A.1
Background InformationHistone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histones are modified post-translationally by acetylation, phosphorylation, methylation, and ubiquitination and these modifications regulate DNA transcription, repair, recombination, and replication. Ubiquitylation usually targets the substrate for degradation, although histones H2A and H2B are actually stabilized by a single ubiquitin conjugation. Histone ubiquitination has been correlated with DNA repair and transcription, cellular differentiation, cell cycle regulation, spermatogenesis, protein trafficking, and response to stress. Histone H2A is one of four components of the core nucleosomal structure. The nucleosome represents a unit of chromatin in which DNA is wrapped around a histone octamer. Histones undergo a number of post-translational modifications (PTM) in response to various stimuli that may induce changes in the structure of the nucleosome and hide or expose DNA sequences. Histone H2A, in particular, undergoes acetylation on lysine 5 by the Tip60 enzyme which may promote unfolding of chromatin and transcription. In addition, H2A is phosphorylated on serine 1 and serine 139 (H2A.X) residues which mark mitosis and gene repression, and DNA damage, respectively. Gene repression may also result from the sumoylation of H2A’s lysine 126 residue. In addition, ubiquitination of the H2A lysine 119 residue may play a role in spermatogenesis. It is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Histone H2A using this rabbit polyclonal antibody, Anti-phospho Histone H2A (Ser129) Antibody validated for use in western blotting, Dot Blot, Peptide Inhibition Assay, IP & ICC.
Key Applications
  • Western Blotting
  • Dot Blot
  • Peptide Inhibition Assay
  • Immunoprecipitation
  • Immunocytochemistry
Application NotesDot Blot (Specificity) Analysis: 0.05 µg/mL from a representative lot detected phospho Histone H2A (Ser129) in various panels of modified and non-modified Histones.

Immunoprecipitation Analysis: A 1:100 dilution from a represenetative lot immunoprecipitated phospho Histone H2A (Ser129) from yeast extracts.

Immunohistochemistry Analysis: A 1:1,000 dilution from a lot detected phospho Histone H2A (Ser129) from yeast cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of yeast Histone H2A phosphorylated at Ser129.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis antibody is specifically recognizes yeast phospho-Histone H2A (Ser129)
Species Reactivity
  • Yeast
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • HTA1
  • H2A1
  • SPT11
Modifications
  • Phosphorylation
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~16 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in untreated and Methyl methanesulfonate (MMS) treated yeast nuclear extract.

Western Blotting Analysis: 0.02 µg/mL of this antibody detected an increase in signal for phospho Histone H2A (Ser129) in MMS treated yeast nuclear extract over untreated yeast nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
07-745-I 04053252935893

Documentation

Anti-phospho Histone H2A (Ser129) Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-phospho Histone H2A (Ser129) Antibody Certificates of Analysis

TitleLot Number
Anti-phospho Histone H2A (Ser129) 2485988
Anti-phospho Histone H2A (Ser129) - 3308254 3308254
Anti-phospho Histone H2A (Ser129) - 3671316 3671316
Anti-phospho Histone H2A (Ser129) - 4013894 4013894
Anti-phospho Histone H2A (Ser129) - Q2315818 Q2315818
Anti-phospho Histone H2A (Ser129) -2764889 2764889
Anti-phospho Histone H2A (Ser129) Polyclonal Antibody 3147029
Anti-phospho Histone H2A (Ser129) Polyclonal Antibody 3054707