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MABS829 Anti-phospho-EGFR (Tyr1173) Antibody, clone 3E7.2

Detect phospho EGFR using this mouse monoclonal Anti-phospho-EGFR (Tyr1173), clone 3E7.2, Cat. No. MABS829, used in Immunocytochemistry and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: EGFR, ERBB, ERBB1, HER1 UniProt Number: P00533
MABS829
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICC, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS829
Replaces05-483
DescriptionAnti-phospho-EGFR (Tyr1173) Antibody, clone 3E7.2
Alternate Names
  • Epidermal growth factor receptor
  • EC: 2.7.10.1
  • Proto-oncogene c-ErbB1
  • Receptor tyrosine protein kinase erbB-1
Background InformationEpidermal growth factor receptor (UniProt: P00533; also known as EC: 2.7.10.1; Proto-oncogene c-ErbB1, Receptor tyrosine protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human. EGFR is activated by binding of its specific ligands and transforms from a monomeric to active homodimeric form. In addition to forming homodimers, EGFR may pair with another member of the ErbB receptor family, such as ErbB2/Her2/neu, to create an activated heterodimer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of five tyrosine residues (Y992, Y1045, Y1068, Y1148 and Y1173) occurs in the C-terminal domain. This autophosphorylation elicits downstream activation and signaling by several other proteins that associate with the phosphorylated tyrosines through their own phosphotyrosine-binding domains. EGFR activation results in at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLC gamma-PKC and STATs modules. These downstream events modulate several cell functions, including migration, adhesion, and proliferation. Endocytosis and inhibition of the activated EGFR by phosphatases constitute key regulatory mechanisms.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect phospho EGFR using this mouse monoclonal Anti-phospho-EGFR (Tyr1173), clone 3E7.2, Cat. No. MABS829, used in Immunocytochemistry and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunocytochemistry Analysis: 10 µg/mL from a representative lot detected phospho-EGFR (Tyr1173) in A431 cells treated with EGF.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 13 amino acids surrrounding Tyrosine 1173 of human epidermal growth factor receptor.
Clone3E7.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • EGFR
  • ERBB
  • ERBB1
  • HER1
Modifications
  • Phosphorylation
Purification MethodProtein G purified
UniProt Number
Molecular Weight~200 kDa observed; 134.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cells treated with EGF.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho-EGFR (Tyr1173) in 10 µg lysate from A431 cells treated with EGF.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS829 04054839168666