It appears that your browser has JavaScript disabled.

This Website requires your browser to be JavaScript enabled.

Please enable JavaScript and reload this page.

Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.

07-1840 Sigma-AldrichAnti-phospho-Acinus (Ser422) Antibody

Use Anti-phospho-Acinus (Ser422) Antibody (rabbit polyclonal antibody) validated in WB to detect phospho-Acinus (Ser422) also known as apoptotic chromatin condensation inducer 1, functional spliceosom

More>>

Research Category: Epigenetics & Nuclear Function, Epigenetics & Nuclear Function Research Sub-Category: Apoptosis - Additional, Chromatin Biology Gene Symbol: ACIN1, ACINUS, CAN, Acinus UniProt Number: Q9UKV3

Recommended Products

Overview

Special Offers

100% Performance Guaranteed

Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
R, H, M	WB	Rb	Serum	Polyclonal Antibody

Description
Catalogue Number	07-1840
Description	Anti-phospho-Acinus (Ser422) Antibody
Alternate Names	apoptotic chromatin condensation inducer 1 apoptotic chromatin condensation inducer in the nucleus functional spliceosome-associated protein 152
Background Information	Acinus (Apoptotic Chromatin condensation Inducer in the Nucleus) is a ubiquitously expressed nuclear factor essential for apoptotic chromatin condensation. The ~220 kDa acinus protein is cleaved by caspase-3 and an additional unidentified protease. This protelytic cleavage releases a 17 kDa fragment to induce chromatin condensation. Acinus is expressed in three isoforms, AcinusL, AcinusS, and AcinusS' with the two shorter isoforms corresponding to the C-terminal region of the longer form.

Product Information
Format	Serum
Control	NGF treated and untreated PC12 cell lysate
Presentation	Unpurified rabbit polyclonal containing serum with 0.05% sodium azide.

Applications
Application	Use Anti-phospho-Acinus (Ser422) Antibody (rabbit polyclonal antibody) validated in WB to detect phospho-Acinus (Ser422) also known as apoptotic chromatin condensation inducer 1, functional spliceosome-associated protein 152.
Key Applications	Western Blotting

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human Acinus phosphorylated at Ser422.
Epitope	Phosphorylated Ser422
Host	Rabbit
Specificity	Recognizes Acinus phosphorylated at Ser422.
Isotype	IgG
Species Reactivity	Rat Human Mouse
Species Reactivity Note	Demonstrated to react with rat. Predicted to react with human and mouse based on 100% sequence homology.
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NP_055792
Gene Symbol	ACIN1 ACINUS CAN Acinus
Modifications	Phosphorylation
Purification Method	Unpurified
UniProt Number	Q9UKV3
UniProt Summary	FUNCTION: Induces apoptotic chromatin condensation after activation by CASP3. SIZE: 1341 amino acids; 151888 Da SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Ubiquitous. PTM: Undergoes proteolytic cleavage; the processed form is active, contrary to the uncleaved form. SIMILARITY: Contains 1 SAP domain.
Molecular Weight	The molecular weight of phospho-Acinus-L is ~ 220 kDa, phospho-Acinus-S' and S are ~ 100 kDa. The caspase degraded form is approximately 58 kDa.

Product Usage Statements
Quality Assurance	Evaluated by Western Blot in NGF treated and untreated PC12 cell lysate. Western Blot Analysis:1:1,000 dilution of this antibody detected Acinus on 10 µg of NGF treated and untreated PC12 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Packaging Information
Material Size	100 µL

Global Trade Item Number
Catalogue Number	GTIN
07-1840	04053252274015

Recently Viewed