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MABT1333-25UL Anti-pan-Actin Antibody, clone 2A3

Anti-pan-Actin, clone 2A3, Cat. No. MABT1333, is a mouse monoclonal antibody that detects all forms of actin and has been tested for use in Western Blotting.

Anti-pan-Actin Antibody, clone 2A3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: ACTA1, ACTA2, ACTB, ACTC1, ACTG1, ACTG2 UniProt Number: P60709
MABT1333-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, Yeast, Mk, H, Ht, Dr, RWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABT1333-25UL
DescriptionAnti-pan-Actin Antibody, clone 2A3
Alternate Names
  • Skeletal muscle alpha-actin
  • Smooth muscle alpha-actin
  • Cytoplasmic beta-actin
  • Cardiac muscle alpha-actin
  • Cytoplasmic gamma-actin
  • Smooth muscle gamma-actin
Background InformationActin is a multi-functional cytoskeletal protein that serves as the basic building block of cytoskeletal microfilaments. Six different actin types have been reported. Skeletal muscle alpha-actin (UniProt: P68133) is encoded by the ACTA1 gene, smooth muscle alpha-actin (UniProt: P62736) by the ACTA2 gene, cytoplasmic beta-actin (UniProt: P60709) by the ACTB gene, cardiac muscle alpha-actin (UniProt: P68032) by the ACTC1 gene, cytoplasmic gamma-actin (UniProt: P63261) by the ACTG1 gene, and smooth muscle gamma-actin (UniProt: P63267) by the ACTG2 (also known as ACTA3) gene. Although actins show about 90% overall sequence homology, isoforms do show spatial, temporal, and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic beta and gamma actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. This pan-actin antibody detects all isoforms actin.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-pan-Actin, clone 2A3, Cat. No. MABT1333, is a mouse monoclonal antibody that detects all forms of actin and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:50 dilution from a representative lot detected Actins in various species samples (e.g. Human, rat, mouse, monkey, Hamster, Drosophila, Yeast) (Courtesy of Martina Mitterhuber, M.sc and Egon Ogris, M.D., Medical University of Vienna, Austria).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a fragment from the C-terminal region of human Actin.
Clone2A3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 2A3 detects all forms of actin in multiple species. It targets an epitope with in the C-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Yeast
  • Monkey
  • Human
  • Hamster
  • Drosophila
  • Rat
Species Reactivity NoteHuman, Monkey, Mouse, Rat, Yeast, Drosophila, Hamster.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • ACTA1
  • ACTA2
  • ACTB
  • ACTC1
  • ACTG1
  • ACTG2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~42 kDa observed; 41.74 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Actins in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT1333-25UL 04054839557965