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NE1024 Anti-p75 Neurotrophin Receptor Rabbit pAb

This Anti-p75 Neurotrophin Receptor Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of p75 Neurotrophin Receptor.

Anti-p75 Neurotrophin Receptor Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
NE1024
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, RRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~75 kDa neurotrophin receptor (p75NTR) protein in cultured mouse fibroblasts stably transfected with human p75NTR. Also recognizes additional lower MW proteins that are cleavage products of p75NTR.
Catalogue NumberNE1024
Brand Family Calbiochem®
Application Data
Detection of human p75 neurotrophin receptor by immunoblotting. Samples: Whole cell lysate (30 µg) from mouse fibroblasts stably tranfected with human p75NTR (Lane 1) and HEK293 cells transiently transfected with human p75NTR (Lane 2). Primary antibody: Anti-p75 Neurotrophin Receptor Rabbit pAb (Cat. No. NE1024) (1-2 µg/ml). Detection: chemiluminescence.
References
ReferencesKanning, K.C., et al. 2003. J. Neurosci. 23, 5425.
Product Information
FormLiquid
FormulationIn PBS.
Positive controlMouse fibroblasts stably transfected with human p75NTR
Preservative0.03% thimerosal
Applications
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Application NotesImmunoblotting (1-2 µg/ml)
Immunocytochemistry (1-5 µg/ml)
Application CommentsThe immunogen sequence comprises the C-terminal intracellular domain. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of amino acids 273-425 of rat p75NTR
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Mercury prohibited To comply with ban of sale of mercury-added products required by The Interstate Mercury Education and Reduction Clearinghouse (IMERC), this product is prohibited to be sold in the following US states: Rhode Island and Connecticut.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
NE1024 0

Documentation

Anti-p75 Neurotrophin Receptor Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-p75 Neurotrophin Receptor Rabbit pAb Certificates of Analysis

TitleLot Number
NE1024

References

Reference overview
Kanning, K.C., et al. 2003. J. Neurosci. 23, 5425.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-May-2011 JSW
ApplicationImmunoblotting (1-2 µg/ml)
Immunocytochemistry (1-5 µg/ml)
Application Data
Detection of human p75 neurotrophin receptor by immunoblotting. Samples: Whole cell lysate (30 µg) from mouse fibroblasts stably tranfected with human p75NTR (Lane 1) and HEK293 cells transiently transfected with human p75NTR (Lane 2). Primary antibody: Anti-p75 Neurotrophin Receptor Rabbit pAb (Cat. No. NE1024) (1-2 µg/ml). Detection: chemiluminescence.
DescriptionPurified rabbit polyclonal antibody. Recognizes the ~75 kDa p75 neurotrophin receptor protein.
BackgroundNeurotrophins are a family of proteins that interact with neurotrophin receptors and provide various survival signals in nervous tissue. p75 neurotrophin receptor (p75NTR) is also known as the low-affinity neurotrophin receptor, while TrkA, TrkB, and TrkC are the high affinity receptors. p75NTR is a member of the tumor necrosis factor receptor family and is common to all neurotrophins. Similar to the high affinity receptors, p75NTR will bind the neurotrophins, but unlike the Trk proteins, it has no cytoplasmic tyrosine kinase domain. The roles of this receptor are controversial, as it may also be involved in either promoting or downregulating the response to the neurotrophins. It may also function to increase the affinity of the trk receptors for their respective neurotrophins or it may bind neurotrophins and prevent them from binding to the high affinity receptors. p75NTR does not have a catalytic intracellular tyrosine kinase domain, but it is still capable of mediating the neurotrophin signals.
HostRabbit
Immunogen speciesRat
Immunogena recombinant protein consisting of amino acids 273-425 of rat p75NTR
IsotypeIgG
Specieshuman, rat
Positive controlMouse fibroblasts stably transfected with human p75NTR
FormLiquid
FormulationIn PBS.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative0.03% thimerosal
CommentsThe immunogen sequence comprises the C-terminal intracellular domain. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesKanning, K.C., et al. 2003. J. Neurosci. 23, 5425.