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OP33 Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620)

This Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-5) (Wild type).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP33
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, H, M, Pm, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP33-100UG Plastic ampoule 100 μg
OP33-20UG Plastic ampoule 20 μg
Description
OverviewRecognizes the ~53 kDa wild-type p53 protein in its native conformation in Hs27 cells and breast carcinoma tissue. Does not recognize mutant or denatured p53 protein.
Catalogue NumberOP33
Brand Family Calbiochem®
References
ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
Ball, R.K., et al. 1984. EMBO J. 3, 1485.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Positive controlHs27 cells or breast carcinoma tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoprecipitation Lu, X., et al. 1992. Cell 70, 153. Frozen Sections Kramata, P., et al. 2005. Cancer Res. 65, 3577. Cordon-Cardo, C., et al. 1991. Cancer Res. 54, 794.
Key Applications Frozen Sections
Immunofluorescence
Immunoprecipitation
Not Western Blot
Paraffin Sections
Application NotesFrozen Sections (see applications references)
Immunoblotting (not recommended)
Immunofluorescence (1-20 μg/ml)
Immunoprecipitation (1 μg per sample)
Paraffin Sections (5 μg/ml, heat pre-treatment required)
Application CommentsWild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met(≤ 1 hr) will help reduce background. p53 (Ab-5) may also be used to visualize p53 in cytospins or cultured cells using standard horseradish peroxidase or immunofluorescence detection techniques. p53 (Ab-5) preferentially immunoprecipitates wild-type p53; it should not precipitate mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenvLM mouse tumor cells
ImmunogenMouse
ClonePAb1620
HostMouse
IsotypeIgG2a
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Primate
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP33-100UG 04055977208443
OP33-20UG 07790788053901

Documentation

Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) Certificates of Analysis

TitleLot Number
OP33

References

Reference overview
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
Ball, R.K., et al. 1984. EMBO J. 3, 1485.

Citations

Title
  • Pavel Kramata, et al. (2005) Patches of Mutant p53-Immunoreactive Epidermal Cells Induced by Chronic UVB Irradiation Harbor the Same p53 Mutations as Squamous Cell Carcinomas in the Skin in Hairless SKH-1 Mice. Cancer Research 65, 3577-3585.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision02-October-2007 RFH
    ApplicationFrozen Sections (see applications references)
    Immunoblotting (not recommended)
    Immunofluorescence (1-20 μg/ml)
    Immunoprecipitation (1 μg per sample)
    Paraffin Sections (5 μg/ml, heat pre-treatment required)
    DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 myeloma cells. Recognizes the ~53 kDa wild-type p53 protein.
    BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phospho-protein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
    HostMouse
    Immunogen speciesMouse
    ImmunogenvLM mouse tumor cells
    ClonePAb1620
    IsotypeIgG2a
    Speciesbovine, human, mouse, primate, rat
    Positive controlHs27 cells or breast carcinoma tissue
    FormLiquid
    FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsWild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met(≤ 1 hr) will help reduce background. p53 (Ab-5) may also be used to visualize p53 in cytospins or cultured cells using standard horseradish peroxidase or immunofluorescence detection techniques. p53 (Ab-5) preferentially immunoprecipitates wild-type p53; it should not precipitate mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Toxicity Standard Handling
    ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
    Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
    Barak, Y., et al. 1993. EMBO J. 12, 461.
    Kastan, M.B., et al. 1992. Cell 71, 587.
    Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
    Lane, D.P. 1992. Nature 358, 15.
    Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
    Ball, R.K., et al. 1984. EMBO J. 3, 1485.
    Citation
  • Pavel Kramata, et al. (2005) Patches of Mutant p53-Immunoreactive Epidermal Cells Induced by Chronic UVB Irradiation Harbor the Same p53 Mutations as Squamous Cell Carcinomas in the Skin in Hairless SKH-1 Mice. Cancer Research 65, 3577-3585.
    		•
    Application referencesImmunoprecipitation Lu, X., et al. 1992. Cell 70, 153. Frozen Sections Kramata, P., et al. 2005. Cancer Res. 65, 3577. Cordon-Cardo, C., et al. 1991. Cancer Res. 54, 794.