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OP32 Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246)

This Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246) is validated for use in Immunocytochemistry, Paraffin Sections, Immunoblotting for the detection of p53 (Ab-4) (Wild type).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP32
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
OverviewRecognizes the ~53 kDa wild-type p53 protein in its native conformation in SV-T2 cells. Does not recognize mutant or denatured p53 protein.

This product has been discontinued.
Catalogue NumberOP32
Brand Family Calbiochem®
Application Data
Detection of mouse p53 by immunocytochemical staining. Sample: SV-T2 cells fixed in methanol. Primary antibody: Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246) (Cat. No. OP32) (1 µg/ml). Detection: fluorescence with DAPI counterstain.
References
ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994.Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative controlSK-OV-3 cells
Positive controlSV-T2 cells
Preservative≤0.1% sodium azide
Applications
Application ReferencesOriginal Clone Yewdell, J.W., et al. 1986. J. Virol. 59, 444.
Key Applications Immunocytochemistry
Immunoprecipitation
Not Western Blot
Paraffin Sections
Application NotesImmunocytochemistry (1-20 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (1-5 µg/ml, heat pre-treatment required)
Immunoblotting (not recommended)
Application CommentsWild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met (less than 1 h) will help reduce background. Anti-p53 (Ab-4) (Wild type), preferentially recognizes wild-type p53 of murine cellular origin; it will not react with mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
Biological Information
ImmunogenBALB/c SVA31 E7 cells
ImmunogenMouse
Epitopea conformational epitope within amino acids 85-109
ClonePAb246
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP32 0

Documentation

Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246) Certificates of Analysis

TitleLot Number
OP32

References

Reference overview
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994.Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-October-2007 RFH
ApplicationImmunocytochemistry (1-20 µg/ml)
Immunoprecipitation (1 µg/sample)
Paraffin Sections (1-5 µg/ml, heat pre-treatment required)
Immunoblotting (not recommended)
Application Data
Detection of mouse p53 by immunocytochemical staining. Sample: SV-T2 cells fixed in methanol. Primary antibody: Anti-p53 (Ab-4) (Wild type) Mouse mAb (PAb246) (Cat. No. OP32) (1 µg/ml). Detection: fluorescence with DAPI counterstain.
DescriptionPurified mouse monoclonal antibody generated by immunizing C57/BL mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 mouse myeloma cells (see application references). Recognizes the ~53 kDa wild-type p53 protein.
BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phospho-protein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1, and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
HostMouse
Immunogen speciesMouse
ImmunogenBALB/c SVA31 E7 cells
Epitopea conformational epitope within amino acids 85-109
ClonePAb246
IsotypeIgG₁
Speciesnot human, mouse, rat
Positive controlSV-T2 cells
Negative controlSK-OV-3 cells
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsWild-type p53 has a short half-life and is present in low amounts in cells. Increasing the amount of sample to be immunoprecipitated and applied to the gel may help for visualization. Short incubation times with 35S-Met (less than 1 h) will help reduce background. Anti-p53 (Ab-4) (Wild type), preferentially recognizes wild-type p53 of murine cellular origin; it will not react with mutant or denatured p53. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994.Cancer Res. 54, 4855.
Barak, Y., et al. 1993. EMBO J. 12, 461.
Lane, D.P. 1992. Nature 358, 15.
Kastan, M.B., et al. 1991. Cancer Res. 51, 6304.
Kastan, M.B., et al. 1992. Cell 71, 587.
Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
Application referencesOriginal Clone Yewdell, J.W., et al. 1986. J. Virol. 59, 444.