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OP104L Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802)

This Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) is validated for use in Immunoblotting, IP, Frozen Sections, IF Paraffin Sections for the detection of p53 (Ab-11) (Pantropic).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP104L
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP104L-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~53 kDa wild-type and mutant p53 protein in immunoblotting. Recognizes only the wild-type p53 protein in immunoprecipitation.
Catalogue NumberOP104L
Brand Family Calbiochem®
References
ReferencesLevine, A.J. 1997. Cell 88, 323.
Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
Waldman, T., et al. 1995. Cancer Res. 55, 5187.
Clarke, A.R., et al. 1994. Oncogene 9, 1767.
Cho, Y., et al. 1994. Science 265, 346.
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Lane, D.P. 1992. Nature 358, 15.
Product Information
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
Negative controlSK-OV-3 cells
Positive controlA431 or SK-BR-3 cells
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting, Immunoprecipitation Banks, L., et al. 1986. Eur. J. Biochem. 159, 529.
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Not Frozen Sections
Not Immunofluorescence
Not Paraffin Sections
Application NotesImmunoblotting (2.5 µg/ml, chemiluminescence, see application references)
Immunoprecipitation (1 µg/reaction, see comments, see application references)
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Paraffin Sections (not recommended)
Application CommentsThis antibody will immunoprecipitate wild-type p53 only. This antibody will stain methanol-fixed cells. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human p53 protein
ImmunogenHuman
Epitopewithin amino acids 306-393 of human p53
ClonePAb1802
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included. Lyophilized antibodies should be reconstituted at 4°C by gradual addition of solvent over a period of 1-2 h with occasional gentle mixing.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP104L-100UG 04055977224917

Documentation

Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) SDS

Title

Safety Data Sheet (SDS) 

Anti-p53 (Ab-11) (Pantropic) Mouse mAb (PAb1802) Certificates of Analysis

TitleLot Number
OP104L

References

Reference overview
Levine, A.J. 1997. Cell 88, 323.
Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
Waldman, T., et al. 1995. Cancer Res. 55, 5187.
Clarke, A.R., et al. 1994. Oncogene 9, 1767.
Cho, Y., et al. 1994. Science 265, 346.
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Lane, D.P. 1992. Nature 358, 15.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision01-October-2007 RFH
ApplicationImmunoblotting (2.5 µg/ml, chemiluminescence, see application references)
Immunoprecipitation (1 µg/reaction, see comments, see application references)
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Paraffin Sections (not recommended)
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~53 kDa wild-type and mutant forms of p53.
Backgroundp53 (393 amino acids) exhibits sequence-specific DNA binding, directly interacts with various cellular and viral proteins, and induces cell cycle arrest in response to DNA damage by inducing GADD45, WAF1, and MDM2. p53 is the most commonly mutated gene in human cancer with most mutations occurring in the DNA contact residues. p53 binds damaged DNA non-specifically via its C-terminal 75 amino acids which in turn may be responsible for subsequent activation, sequence-specific DNA binding, and WAF1 induction allowing DNA repair to take place. p53 down regulates bcl-2 expression and up regulates bax expression, but may not always be necessary for apoptosis. In addition to the afore mentioned proteins, two other proteins involved in replication and recombination, RPA and RAD51, also bind p53.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant, human p53 protein
Epitopewithin amino acids 306-393 of human p53
ClonePAb1802
IsotypeIgG₁
Specieshuman, mouse
Positive controlA431 or SK-BR-3 cells
Negative controlSK-OV-3 cells
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
PreservativeNone
CommentsThis antibody will immunoprecipitate wild-type p53 only. This antibody will stain methanol-fixed cells. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included. Lyophilized antibodies should be reconstituted at 4°C by gradual addition of solvent over a period of 1-2 h with occasional gentle mixing.
Toxicity Standard Handling
ReferencesLevine, A.J. 1997. Cell 88, 323.
Miller, S.D., et al. 1997. Mol. Cell. Biol. 17, 2194.
Pellegata, N.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 15209.
Sturzbecher, H.-W., et al. 1996. EMBO J. 15, 1992.
Argarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
Götz, C. and Montenarh, M. 1995. Int. J. Oncology 6, 1129.
Reed, M. et al. 1995. Proc. Natl. Acad. Sci. USA 92, 9455.
Waldman, T., et al. 1995. Cancer Res. 55, 5187.
Clarke, A.R., et al. 1994. Oncogene 9, 1767.
Cho, Y., et al. 1994. Science 265, 346.
El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
Lane, D.P. 1992. Nature 358, 15.
Application referencesImmunoblotting, Immunoprecipitation Banks, L., et al. 1986. Eur. J. Biochem. 159, 529.