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OP64F Anti-p21WAF1 (58-77) (Ab-1) Mouse mAb (EA10) Fluorescein Conjugate

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-SD11, Anti-p21, Anti-WAF, Anti-CIP1

OP64F
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~21 kDa p21WAF1 protein in skin and colon tissue and in cells expressing wild-type p53 (e.g. Hs27 or U205 cells treated with DNA damaging agents).
Catalogue NumberOP64F
Brand Family Calbiochem®
SynonymsAnti-SD11, Anti-p21, Anti-WAF, Anti-CIP1
References
ReferencesAgarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology 7, 889.
Deng, C., et al. 1995. Cell 82, 675.
Waldman, T., et al. 1995. Cancer Res. 55, 5187.
Elbendary, A., et al.1994. Cell Growth Diff. 5, 1301.
El-Deiry, W.S., et al. 1994 Cancer Res. 54, 1169.
Li, R., et al. 1994. Nature 371, 534.
Michieli, P., et al. 1994. Cancer Res. 54, 3391.
Noda, A., et al.1994. Exp. Cell Res. 211, 90.
El-Deiry, W.S., et al.1993. Cell 75, 817.
Gu, Y., et al. 1993. Nature 366, 707.
Harper, J.W., et al.1993. Cell 75, 805.
Xiong, Y., et al.1993. Genes Devel. 7, 1572.
Xiong, Y., et al.1993. Nature 366, 701.
Xiong, Y., et al.1992. Cell 71, 505.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
Negative controlUnstimulated cells, unstimulated skin tissue, or SK-OV-3 cells
Positive controlAny cell line expressing wild-type p53 (e.g. Hs27 cells or U2OS cells treated with DNA damaging agents) or UV-treated skin or colon tissue
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesParaffin Sections, Frozen Sections, Original Clone El-Deiry, W.S., et al. 1995. Cancer Res. 55, 2910. Epitope Identification Patrick O'Connor (NCI, personal communication).
Key Applications Flow Cytometry
Immunofluorescence
Application NotesFlow Cytometry (2 µg/ml)
Frozen Sections (5 µg/ml)
Immunoblotting (use Cat. No. OP64)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (use Cat. No. OP64)
Paraffin Sections (5 µg/ml, see comments)
Application CommentsMaximal p21WAF1 expression requires wild type p53 activity. Treatment of U2OS or MCF7 cells with DNA damaging agents (such as doxorubicin at 0.2 µg/ml) induces wild type p53 expression which in turn activates p21WAF1 expression. Serum stimulation of quiescent cells will give low level p21WAF1 expression independent of p53 expression. Untreated cells will express little p21WAF1 and can also be used as a negative control. Cat. No. OP64F was tested in HALT cells induced by incubation at 31°C; FITC-goat anti-mouse IgG (Cat. No. DC13L) was used as a negative control. For staining paraffin sections, no pretreatment is usually needed, but some tissues may stain better after pretreating with heat or pressure cooker. In either paraffin or frozen sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21WAF1 while the proliferating compartment of crypts will not stain. For immunoprecipitation and immunoblotting use Cat. No. OP64. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenfull-length, recombinant, human p21WAF1
ImmunogenHuman
Epitopewithin amino acids 58-77 of human p21WAF1
CloneEA10
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP64F 0

Documentation

Anti-p21WAF1 (58-77) (Ab-1) Mouse mAb (EA10) Fluorescein Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-p21WAF1 (58-77) (Ab-1) Mouse mAb (EA10) Fluorescein Conjugate Certificates of Analysis

TitleLot Number
OP64F

References

Reference overview
Agarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology 7, 889.
Deng, C., et al. 1995. Cell 82, 675.
Waldman, T., et al. 1995. Cancer Res. 55, 5187.
Elbendary, A., et al.1994. Cell Growth Diff. 5, 1301.
El-Deiry, W.S., et al. 1994 Cancer Res. 54, 1169.
Li, R., et al. 1994. Nature 371, 534.
Michieli, P., et al. 1994. Cancer Res. 54, 3391.
Noda, A., et al.1994. Exp. Cell Res. 211, 90.
El-Deiry, W.S., et al.1993. Cell 75, 817.
Gu, Y., et al. 1993. Nature 366, 707.
Harper, J.W., et al.1993. Cell 75, 805.
Xiong, Y., et al.1993. Genes Devel. 7, 1572.
Xiong, Y., et al.1993. Nature 366, 701.
Xiong, Y., et al.1992. Cell 71, 505.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-October-2007 RFH
SynonymsAnti-SD11, Anti-p21, Anti-WAF, Anti-CIP1
ApplicationFlow Cytometry (2 µg/ml)
Frozen Sections (5 µg/ml)
Immunoblotting (use Cat. No. OP64)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (use Cat. No. OP64)
Paraffin Sections (5 µg/ml, see comments)
DescriptionPurified mouse monoclonal antibody generated by immunizing F1 mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes the ~21 kDa p21WAF1 protein.
BackgroundThe tumor suppressor p53 transcriptionally activates a number of genes including the WAF1/CIP1 gene in response to DNA damage. The 21 kDa product of the WAF1 gene is found in a complex involving cyclins, cyclin dependent kinases (CDK), and PCNA in normal cells but not transformed cells and appears to be a universal inhibitor of CDK activity. One consequence of p21WAF1 binding to and inhibiting CDKs is to prevent CDK-dependent phosphorylation and subsequent inactivation of the Rb protein which is essential for cell cycle progression. p21WAF1 is, therefore, a potent and reversible inhibitor of cell cycle progression at both the G1 and G2 checkpoints, presumably to allow sufficient time for DNA repair to be completed. Irreversible G1 or G2 arrest leads to apoptosis. However, the role of p21WAF1 in apoptosis is less clear although p53-mediated apoptosis leads to increased WAF1 expression. Induction of p21WAF1 in response to DNA damage can occur by both p53-dependent and p53-independent mechanisms, in response to mitogenic stimuli, differentiation, or in tumor cells with mutated p53. Functional p21WAF1 is essential for p53-mediated G1 arrest presumably due to WAF1 inhibition of both CDK activity and PCNA-dependent DNA replication. WAF1 has also been identified as a gene involved in cellular senescence, termed sdi1. Not surprisingly, p21WAF1 overexpression is growth suppressive, consistent with its role as an inhibitor of CDKs. By inhibiting Rb inactivation in a p53-dependent fashion, p21WAF1 serves to integrate cell cycle control mediated by p53 and Rb.
HostMouse
Immunogen speciesHuman
Immunogenfull-length, recombinant, human p21WAF1
Epitopewithin amino acids 58-77 of human p21WAF1
CloneEA10
IsotypeIgG₁
Specieshuman, not mouse, not rat
Positive controlAny cell line expressing wild-type p53 (e.g. Hs27 cells or U2OS cells treated with DNA damaging agents) or UV-treated skin or colon tissue
Negative controlUnstimulated cells, unstimulated skin tissue, or SK-OV-3 cells
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsMaximal p21WAF1 expression requires wild type p53 activity. Treatment of U2OS or MCF7 cells with DNA damaging agents (such as doxorubicin at 0.2 µg/ml) induces wild type p53 expression which in turn activates p21WAF1 expression. Serum stimulation of quiescent cells will give low level p21WAF1 expression independent of p53 expression. Untreated cells will express little p21WAF1 and can also be used as a negative control. Cat. No. OP64F was tested in HALT cells induced by incubation at 31°C; FITC-goat anti-mouse IgG (Cat. No. DC13L) was used as a negative control. For staining paraffin sections, no pretreatment is usually needed, but some tissues may stain better after pretreating with heat or pressure cooker. In either paraffin or frozen sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21WAF1 while the proliferating compartment of crypts will not stain. For immunoprecipitation and immunoblotting use Cat. No. OP64. Antibody should be titrated for optimal results in individual systems.
Storage Protect from light
Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesAgarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
Chen, Y.Q., et al. 1995. Int. J. Oncology 7, 889.
Deng, C., et al. 1995. Cell 82, 675.
Waldman, T., et al. 1995. Cancer Res. 55, 5187.
Elbendary, A., et al.1994. Cell Growth Diff. 5, 1301.
El-Deiry, W.S., et al. 1994 Cancer Res. 54, 1169.
Li, R., et al. 1994. Nature 371, 534.
Michieli, P., et al. 1994. Cancer Res. 54, 3391.
Noda, A., et al.1994. Exp. Cell Res. 211, 90.
El-Deiry, W.S., et al.1993. Cell 75, 817.
Gu, Y., et al. 1993. Nature 366, 707.
Harper, J.W., et al.1993. Cell 75, 805.
Xiong, Y., et al.1993. Genes Devel. 7, 1572.
Xiong, Y., et al.1993. Nature 366, 701.
Xiong, Y., et al.1992. Cell 71, 505.
Application referencesParaffin Sections, Frozen Sections, Original Clone El-Deiry, W.S., et al. 1995. Cancer Res. 55, 2910. Epitope Identification Patrick O'Connor (NCI, personal communication).