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MABN92-25UG Anti-lba1/AIF1 (mouse monoclonal)

Anti-Iba1/AIF1 Antibody is an antibody against Iba1/AIF1 for use in WB, IH.

MABN92-25UG
25 μg  
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Description
Catalogue NumberMABN92-25UG
DescriptionAnti-lba1/AIF1 (mouse monoclonal)
Alternate Names
  • Allograft inflammatory factor 1
  • AIF-1
  • Ionized calcium-binding adapter molecule 1
  • Protein G1
Background InformationThe allograft inflammatory factor-1 (AIF-1) is a 17 kDa interferon-gamma (IFN-gamma) inducible Ca(2+)-binding EF-hand protein that is encoded within the HLA class III genomic region. AIF-1 is known to be identical with ionized Ca(2+)-binding adaptor 1, microglia response factor-1, and daintain. AIF-1 expression is mostly limited to the macrophage/monocyte lineage, and is augmented by cytokines, such as IFN-gamma. It is reported to be involved in neuronal function, inflammatory responses, allograft rejection, autoimmunity, and the activation and function of macrophages. It is also shown to be expressed in macrophages and microglial cells in autominnume disesases such as experimental autoimmune encephalomyelitis, and neuritis and uveitis models.
References
Product Information
FormatPurified
Control
  • Human thymus tissue lysate
PresentationPurified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Iba1/AIF1 Antibody is an antibody against Iba1/AIF1 for use in WB, IH.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blot Analysis: 1 µg/mL from a representative lot detected Iba1/AIF1 in 10 µg of mouse thymus tissue lysate.

Western Blot Analysis: 1 µg/mL from a representative lot detected Iba1/AIF1 in 10 µg of rat thymus tissue lysate.

Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected Iba/AIF1 in human spleen tissue.
Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected Iba/AIF1 in rat spleen tissue
Biological Information
ImmunogenLinear peptide corresponding to human Iba1/AIF1.
Clone20A12.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • AIF1
  • G1
  • IBA1
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.

SUBUNIT STRUCTURE: Homodimer. Monomer. Interacts with LCP1.

SUBCELLULAR LOCATION: Cytoplasm > cytoskeleton. Cell projection > ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Associated with the actin cytoskeleton at membrane ruffles and at sites of phagocytosis.

TISSUE SPECIFICITY: Detected in T-lymphocytes and peripheral blood mononuclear cells.
Molecular Weight~17 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated in Western Blot in human thymus tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Iba1/AIF1 in 10 µg of human thymus tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN92-25UG 04054839342769

Documentation

Anti-lba1/AIF1 (mouse monoclonal) SDS

Title

Safety Data Sheet (SDS) 

Anti-lba1/AIF1 (mouse monoclonal) Certificates of Analysis

TitleLot Number
Anti-Rhodopsin, clone 4D2 - 3313746 3313746
Anti-lba1/AIF1 (mouse monoclonal) - 3312992 3312992
Anti-lba1/AIF1 (mouse monoclonal) - 3478259 3478259
Anti-lba1/AIF1 (mouse monoclonal) - 3736631 3736631
Anti-lba1/AIF1 (mouse monoclonal) - 3800109 3800109
Anti-lba1/AIF1 (mouse monoclonal) - 3814317 3814317
Anti-lba1/AIF1 (mouse monoclonal) - 3866800 3866800
Anti-lba1/AIF1 (mouse monoclonal) - 3942763 3942763
Anti-lba1/AIF1 (mouse monoclonal) - 3978860 3978860
Anti-lba1/AIF1 (mouse monoclonal) - 4046558 4046558
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