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PC388 Anti-hMre-11 Rabbit pAb

Anti-hMre-11, rabbit polyclonal, recognizes the ~85 kDa hMre-11 protein in IMR90 and K562 cells. It is validated for use in Western blotting, immunofluorescence, and immunoprecipitation.

Anti-hMre-11 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC388
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~85 kDa hMre-11 protein in IMR90 and K562 cells.
Catalogue NumberPC388
Brand Family Calbiochem®
Application Data
Detection of human hMre11 by immunocytochemistry. Sample: IMR90 primary human fibroblasts left untreated (panel A) or treated with ionizing radiation (panel B). Primary antibody: Anti-hMre-11 Rabbit pAb (Cat. No. PC388T) (1 µg/ml). Detection: fluorescence. Courtesy of R. Maser and J. Petrini.
References
ReferencesDong, Z., et al. 1999. J. Biol. Chem. 274, 19513.
Luo, G., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 7376.
Petrini, J.H. 1999. Am. J. Hum. Genet. 64, 1264.
Carney, J.P., et al. 1998. Cell 93, 477.
Haber, J.E. 1998. Cell 95, 583.
Nelms, B.E., et al. 1998. Science 280, 590.
Maser, R.S., et al. 1997. Mol. Cell Biol. 17, 6087.
Xiao, Y. and D.T. Weaver. 1997. Nucleic Acids Res. 25, 2985.
Dolganov, G.M., et al. 1996. Mol. Cell Biol. 16, 4832.
Petrini, J.H., et al. 1995. Genomics 29, 80.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlIMR90 or K562 cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesImmunoblotting (1 µg/ml)
Immunofluorescence (1 µg/ml)
Immunoprecipitation (1 µg/reaction)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human hMre11 protein
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC388 0

Documentation

Anti-hMre-11 Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-hMre-11 Rabbit pAb Certificates of Analysis

TitleLot Number
PC388

References

Reference overview
Dong, Z., et al. 1999. J. Biol. Chem. 274, 19513.
Luo, G., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 7376.
Petrini, J.H. 1999. Am. J. Hum. Genet. 64, 1264.
Carney, J.P., et al. 1998. Cell 93, 477.
Haber, J.E. 1998. Cell 95, 583.
Nelms, B.E., et al. 1998. Science 280, 590.
Maser, R.S., et al. 1997. Mol. Cell Biol. 17, 6087.
Xiao, Y. and D.T. Weaver. 1997. Nucleic Acids Res. 25, 2985.
Dolganov, G.M., et al. 1996. Mol. Cell Biol. 16, 4832.
Petrini, J.H., et al. 1995. Genomics 29, 80.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-October-2007 RFH
ApplicationImmunoblotting (1 µg/ml)
Immunofluorescence (1 µg/ml)
Immunoprecipitation (1 µg/reaction)
Application Data
Detection of human hMre11 by immunocytochemistry. Sample: IMR90 primary human fibroblasts left untreated (panel A) or treated with ionizing radiation (panel B). Primary antibody: Anti-hMre-11 Rabbit pAb (Cat. No. PC388T) (1 µg/ml). Detection: fluorescence. Courtesy of R. Maser and J. Petrini.
DescriptionAffinity purified rabbit polyclonal antibody. Recognizes the ~85 kDa hMre-11 protein.
BackgroundDouble strand breaks (DSB) in DNA are repaired by an evolutionarily conserved complex that includes the proteins Mre11 and Rad50. Both of these proteins are essential since nullizygous embryos die in utero, consistent with the fact that they both participate in two distinct processes: homologous recombination and non-homologous end joining. The Mre11 gene encodes a 680 amino acid protein that migrates on SDS/with an apparent mobility of ~85 kDa. Among many different functions ascribed to it, Mre11 exhibits a Mn2+-dependent 3'-5' exonuclease activity on double stranded DNA and a single-stranded endonuclease that facilitates repair of double strand breaks in DNA. Activation of Mre11 may involve post-translational modification since hyperphosphorylation is observed upon DNA damage. Efficient repair of DSBs in mammalian cells appears to require formation of nuclear foci comprised of Mre11, Rad50, and p95NBS1. These foci are observed following induction of DSBs with ionizing radiation, and ionizing radiation-induced foci (IRIF) containing Mre11 appear in a radiation dose dependent manner that slowly dissipates over time, presumably after DSB repair is completed. IRIF containing Mre11 do not form in cells lacking p95NBS1; and in cells lacking ATM, the number of IRIF is dramatically reduced. While Mre11's catalytic activites are essential for repair of DSBs, the Mre11/Rad50/p95NBS1 appears to function as a primary detector of DNA damage.
HostRabbit
Immunogen speciesHuman
Immunogenrecombinant, human hMre11 protein
IsotypeIgG
Specieshuman, mouse, rat
Positive controlIMR90 or K562 cells
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesDong, Z., et al. 1999. J. Biol. Chem. 274, 19513.
Luo, G., et al. 1999. Proc. Natl. Acad. Sci. USA 96, 7376.
Petrini, J.H. 1999. Am. J. Hum. Genet. 64, 1264.
Carney, J.P., et al. 1998. Cell 93, 477.
Haber, J.E. 1998. Cell 95, 583.
Nelms, B.E., et al. 1998. Science 280, 590.
Maser, R.S., et al. 1997. Mol. Cell Biol. 17, 6087.
Xiao, Y. and D.T. Weaver. 1997. Nucleic Acids Res. 25, 2985.
Dolganov, G.M., et al. 1996. Mol. Cell Biol. 16, 4832.
Petrini, J.H., et al. 1995. Genomics 29, 80.