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OP10L Anti-c-Myc (Ab-1) Mouse mAb (9E10)

This Anti-c-Myc (Ab-1) Mouse mAb (9E10) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Chromatin IP, Paraffin Sections for the detection of c-Myc (Ab-1).

Anti-c-Myc (Ab-1) Mouse mAb (9E10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
OP10L
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
OP10L-200UG Plastic ampoule 200 μg
Description
OverviewRecognizes a ~60-67 kDa c-Myc protein in HL60 cells and lung carcinoma tissue.
Catalogue NumberOP10L
Brand Family Calbiochem®
References
ReferencesLeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D. 1986. Ann. Rev. Gen. 20, 361.
Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
Nau, M.M., et al. 1985. Nature 318, 69.
Persson, H., et al. 1984. Science 225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
Product Information
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
Negative controlHT1080 cells
Positive controlHL-60 cells or lung carcinoma
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesEpitope Tagging Munro, S., et al. 1986. Cell 46, 291. Immunoblotting Evan, G.I., et al. 1985. Mol. Cell. Biol. 5, 3610. Chromatin Immunoprecipitation Sanyal, K., et al. 2004. Proc. Natl. Acad. Sci. USA 101, 11374.
Key Applications Flow Cytometry
Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Not Paraffin Sections
Application NotesFlow Cytometry (20 µg/ml or use Cat. No. OP10F)
Frozen Sections (2-10 µg/ml)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml or use Cat. No. OP10F)
Immunoprecipitation (1 µg/sample)
Chromatin Immunoprecipitation (5 µg/ml, see application references)
Paraffin Sections (not recommended)
Application CommentsThe c-Myc protein is extremely labile. c-Myc degradation can be as a result of freeze/thaw cycles, thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-Myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. This antibody recognizes the c-Myc protein and its cleavage products. The sequence of c-Myc predicts a ~45 kDa protein, but c-Myc migrates under reducing conditions as a ~64-67 kDa band. This antibody will not detect v-Myc but may react weakly to rodent c-Myc when used at high concentrations (10 µg/ml). An extra ~34-40 kDa band may be detected in a immunoblot. May also be used in immunofluorescence as well as the detection and purification of recombinant proteins tagged with the Myc epitope sequence EQKLISEEDL (see application references). The immunogen, c-Myc (Peptide-1) is also available for competition studies (Cat. No. PP06). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to amino acids 408-439 of human c-Myc
ImmunogenHuman
Epitopewithin amino acids 410-419
Clone9E10
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP10L-200UG 04055977225044

Documentation

Anti-c-Myc (Ab-1) Mouse mAb (9E10) SDS

Title

Safety Data Sheet (SDS) 

Anti-c-Myc (Ab-1) Mouse mAb (9E10) Certificates of Analysis

TitleLot Number
OP10L

References

Reference overview
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D. 1986. Ann. Rev. Gen. 20, 361.
Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
Nau, M.M., et al. 1985. Nature 318, 69.
Persson, H., et al. 1984. Science 225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-October-2007 RFH
ApplicationFlow Cytometry (20 µg/ml or use Cat. No. OP10F)
Frozen Sections (2-10 µg/ml)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml or use Cat. No. OP10F)
Immunoprecipitation (1 µg/sample)
Chromatin Immunoprecipitation (5 µg/ml, see application references)
Paraffin Sections (not recommended)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells. Recognizes the ~64-67 kDa (apparent MW) c-Myc protein.
BackgroundThe v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc, and B-myc. Amplification of the c-myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-myc gene has been found amplified in neuroblastomas, small cell lung carcinomas and Wilms tumors. L-myc is amplified in small cell lung cancer. Immunological studies have shown that the human c-myc gene gives rise to at least two nuclear phosphoproteins of ~64 kDa and ~67 kDa that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
HostMouse
Immunogen speciesHuman
Immunogena synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to amino acids 408-439 of human c-Myc
Epitopewithin amino acids 410-419
Clone9E10
IsotypeIgG₁
Specieshuman, mouse (weakly), rat (weakly)
Positive controlHL-60 cells or lung carcinoma
Negative controlHT1080 cells
FormLyophilized
FormulationLyophilized from a volatile buffer, 100 µg BSA.
PreservativeNone
CommentsThe c-Myc protein is extremely labile. c-Myc degradation can be as a result of freeze/thaw cycles, thus, we recommend using fresh lysates with a cocktail of protease inhibitors. c-Myc is often destroyed during the processing of paraffin sections and can therefore be difficult to detect in this application. This antibody recognizes the c-Myc protein and its cleavage products. The sequence of c-Myc predicts a ~45 kDa protein, but c-Myc migrates under reducing conditions as a ~64-67 kDa band. This antibody will not detect v-Myc but may react weakly to rodent c-Myc when used at high concentrations (10 µg/ml). An extra ~34-40 kDa band may be detected in a immunoblot. May also be used in immunofluorescence as well as the detection and purification of recombinant proteins tagged with the Myc epitope sequence EQKLISEEDL (see application references). The immunogen, c-Myc (Peptide-1) is also available for competition studies (Cat. No. PP06). Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.
Toxicity Standard Handling
ReferencesLeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D. 1986. Ann. Rev. Gen. 20, 361.
Nisen, P.D., et al. 1986. Cancer Res. 46, 6217.
Nau, M.M., et al. 1985. Nature 318, 69.
Persson, H., et al. 1984. Science 225, 687.
Alitalo, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 1707.
Application referencesEpitope Tagging Munro, S., et al. 1986. Cell 46, 291. Immunoblotting Evan, G.I., et al. 1985. Mol. Cell. Biol. 5, 3610. Chromatin Immunoprecipitation Sanyal, K., et al. 2004. Proc. Natl. Acad. Sci. USA 101, 11374.