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PC05L Anti-c-Fos (Ab-2) (4-17) Rabbit pAb

This Anti-c-Fos (Ab-2) (4-17) Rabbit pAb is validated for use in Frozen Sections, WB, IF, IP, Paraffin Sections, Gel Shift, for the detection of c-Fos (Ab-2) (4-17).

Anti-c-Fos (Ab-2) (4-17) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC05L
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RRbPolyclonal Antibody
Description
OverviewRecognizes the ~50-62 kDa c-Fos protein in HepG2, NIH3T3, and MCF-7 cells.
Catalogue NumberPC05L
Brand Family Calbiochem®
References
ReferencesDeTogni, P., et al. 1988. Cell Biol. 8, 2251.
Rouscher III, F.J., et al. 1988. Science 240, 1010.
Sassone-Corsi, P., et al. 1988. Cell 54, 553.
Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
Verma, I. 1986. Trends Genet. 2, 93.
Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433
Product Information
FormLyophilized
FormulationLyophilized from PBS, a volatile buffer, 100 µg BSA.
Positive controlHep G2 or NIH 3T3 cells
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesGel Shift
Guo, R-F., et al. 2002. Am. J. Pathol. 161, 275.
Key Applications Frozen Sections
Gel Shift
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Not Free-floating Sections
Paraffin Sections
Application NotesFrozen Sections (5-10 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg per reaction)
Paraffin Sections (5-10 µg/ml, heat or pepsin pre-treatment required)
Gel Shift (see comments and application references)
Free-floating Sections (not recommended)
Application CommentsFor gel shift assays resuspend in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibodies should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Ok to freeze
Special InstructionsResuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC05L 0

Documentation

Anti-c-Fos (Ab-2) (4-17) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-c-Fos (Ab-2) (4-17) Rabbit pAb Certificates of Analysis

TitleLot Number
PC05L

References

Reference overview
DeTogni, P., et al. 1988. Cell Biol. 8, 2251.
Rouscher III, F.J., et al. 1988. Science 240, 1010.
Sassone-Corsi, P., et al. 1988. Cell 54, 553.
Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
Verma, I. 1986. Trends Genet. 2, 93.
Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433

Citations

Title
  • Abigail Derbyshire and Mike Ludwig. (2004) TFF3 induced Fos protein expression in the magnocellular oxytocin neurons of the hypothalamus. Peptides 25, 833-838.
  • Ren-Feng Guo, et al. (2002) Activator Protein-1 Actvation in Acute Lung Injury. American Journal of Pathology 161, 275-282.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision17-September-2015 JSW
    ApplicationFrozen Sections (5-10 µg/ml)
    Immunoblotting (1-5 µg/ml)
    Immunofluorescence (1-5 µg/ml)
    Immunoprecipitation (1-2 µg per reaction)
    Paraffin Sections (5-10 µg/ml, heat or pepsin pre-treatment required)
    Gel Shift (see comments and application references)
    Free-floating Sections (not recommended)
    DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~50-62 kDa c-Fos protein.
    BackgroundThe proto-oncogene fos has been implicated in cell growth, differentiation and development. fos is induced by a large number of stimuli including mitogens, differentiation-specific agents, pharmacological agents, etc. Induction of the 62,000 dalton fos protein is rapid but transient. The fos protein is associated with a 39,000 dalton protein which has been identified as the protein product of the jun proto-oncogene (c-jun). The fos/jun protein complex binds specifically to a sequence element in DNA referred to as the AP-1 binding site.
    HostRabbit
    Immunogen speciesHuman
    Immunogena synthetic peptide (SGFNADYEASSSRC) (Cat. No. PP10) corresponding to amino acids 4-17 of human c-Fos
    IsotypeIgG
    Specieshuman, mouse, rat
    Positive controlHep G2 or NIH 3T3 cells
    FormLyophilized
    FormulationLyophilized from PBS, a volatile buffer, 100 µg BSA.
    PreservativeNone
    CommentsFor gel shift assays resuspend in 100 µl of buffer. The immunogen, c-fos (Peptide-2), Cat. No. PP10, is also available for competition studies (see Murphy, et al. below). Antibodies should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Ok to freeze
    Special InstructionsResuspend the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing.
    Toxicity Standard Handling
    ReferencesDeTogni, P., et al. 1988. Cell Biol. 8, 2251.
    Rouscher III, F.J., et al. 1988. Science 240, 1010.
    Sassone-Corsi, P., et al. 1988. Cell 54, 553.
    Verma, I.M. and Graham, W.R. 1987. Cancer Res. 49, 29.
    Verma, I.M. and Sassone-Corsi, P. 1987. Cell 51, 513.
    Muller, R. 1986. Biochim. Biophys. Acta 823, 207.
    Verma, I. 1986. Trends Genet. 2, 93.
    Greenberg, M. and Ziff, E. 1984. Nature (London), 311, 433
    Citation
  • Abigail Derbyshire and Mike Ludwig. (2004) TFF3 induced Fos protein expression in the magnocellular oxytocin neurons of the hypothalamus. Peptides 25, 833-838.
  • Ren-Feng Guo, et al. (2002) Activator Protein-1 Actvation in Acute Lung Injury. American Journal of Pathology 161, 275-282.
    		•
    Application referencesGel Shift
    Guo, R-F., et al. 2002. Am. J. Pathol. 161, 275.