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ABT264 Anti-beta Actin Antibody, arginylated (N-terminal)

This Anti-beta Actin Antibody, arginylated (N-terminal) is validated for use in Dot Blot, Immunocytochemistry, and Western Blotting for the detection of beta Actin N-terminal arginylation.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: ACTB UniProt Number: P60709
ABT264
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RDB, ICC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT264
DescriptionAnti-beta Actin Antibody, arginylated (N-terminal)
Alternate Names
  • Actin, cytoplasmic 1, arginylated
  • beta Actin, arginylated
Background InformationActin, cytoplasmic 1 (UniProt P60709; also known as beta-actin, PS1TP5-binding protein 1) is encoded by the ACTB (also known as BRWS1, PS1TP5BP1) gene (Gene ID 60) in human. Post-translational protein arginylation is mediated by Arg–transfer RNA (-tRNA) protein transferase (Ate1; arginyltransferase). β- and γ-actins are the major structural components of the actin cytoskeleton in nonmuscle cells. Despite their similarity, the β-, but not the γ-, actin is N-terminally arginylated in vivo, where the first two N-terminal amino acids are removed and replaced with an arginine (from NH2-MDDDIAAL- to NH2-RDDIAAL-). In the absence of arginylation, actin forms aggregates during cell-free polymerization. Fibroblasts derived from Ate1 knockout (KO) mice show collapsed lamella, which can be rescued by reintroducing N-terminally arginylated β-actin into the Ate1 KO cells. Intracellular actin polymer levels are shown to drastically decrease in arginylation-deficient cells, which results from changes in actin polymerization properties.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-beta Actin Antibody, arginylated (N-terminal) is validated for use in Dot Blot, Immunocytochemistry, and Western Blotting for the detection of beta Actin N-terminal arginylation.
Key Applications
  • Dot Blot
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunocytochemistry Analysis: 2.0 µg/mL from a representative lot detected N-terminally arginylated beta actin in HUVECs and HeLa cells.

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected N-terminally arginylated beta actin in human (HeLa, HEK293, HepG2, HUVEC, Jurkat, PC3), rat (PC-12 pheochromocytoma), and murine (C2C12 myoblast, NIH/3T3, and Raw 264.7) cell lysates, as well as in human placenta and mouse brain tissue homogenates.

Dot Blot Analysis: 0.2 µg/mL from a representative lot detected immunogen peptide, but not the corresponding peptide without the N-terminus arginine (Courtesy of Dr. Anna Kashina, University of Pennsylvania, Philadelphia, PA).

Western Blotting Analysis: 0.2 µg/mL from a representative lot detected greatly reduced beta actin N-terminal arginylation modification in Arg-transfer RNA (Arg-tRNA) protein transferase (Ate1) knockout mouse embryonic fibroblasts (Courtesy of Dr. Anna Kashina, University of Pennsylvania, Philadelphia, PA).

Note: Goat serum is found to interfere with the staining by this polyclonal antibody, BSA is recommended for sample blocking when using this product.
Biological Information
ImmunogenC-terminally KLH-conjugated linear peptide corresponding to human beta-actin N-terminal sequence with arginylation modification.
EpitopeN-terminus.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detects beta-actin posttranslationally modified by the removal of Met1 and Asp 2 and the arginylation of Asp3. This antibody does not react with beta-actin with unmodified N-terminus.
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Rabbit, Canine, Hamster, Orangatan based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ACTB
Purification MethodAffinity purfied.
UniProt Number
Molecular Weight~43 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected N-terminally arginylated beta actin in human A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABT264 04055977288056

Documentation

Anti-beta Actin Antibody, arginylated (N-terminal) SDS

Title

Safety Data Sheet (SDS) 

Anti-beta Actin Antibody, arginylated (N-terminal) Certificates of Analysis

TitleLot Number
Anti-beta Actin, arginylated (N-terminal) - 3189706 3189706
Anti-beta Actin, arginylated (N-terminal) - 3384844 3384844
Anti-beta Actin, arginylated (N-terminal) - 3991542 3991542
Anti-beta Actin, arginylated (N-terminal) - 4023278 4023278
Anti-beta Actin, arginylated (N-terminal) Polyclonal Antibody 2943577
Anti-beta Actin, arginylated (N-terminal) Polyclonal Antibody 3158272
Anti-beta Actin, arginylated (N-terminal) Polyclonal Antibody 3068060
Anti-beta Actin, arginylated (N-terminal) Polyclonal Antibody 2860593
Anti-beta Actin, arginylated -Q2608332 Q2608332