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MABS1697 Anti-WISP-3 Antibody, clone 13C4.1

Detect WISP-3 using this mouse monoclonal Anti-WISP-3, clone 13C4.1, Cat. No. MABS1697, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-WISP-3 Antibody, clone 13C4.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: WISP3, CCN6, LIBC, PPAC, PPRD, UNQ462/PRO790/PRO956 UniProt Number: O95389
MABS1697
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, H, MWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1697
DescriptionAnti-WISP-3 Antibody, clone 13C4.1
Alternate Names
  • WNT1-inducible-signaling pathway protein 3
  • CCN family member 6
  • WISP-3
Background InformationWNT1-inducible-signaling pathway protein 3 (UniProt O95389; also known as CCN family member 6, WISP-3) is encoded by the WISP3 (also known as CCN6, LIBC, PPAC, PPRD, UNQ462/PRO790/PRO956) gene (Gene ID 8838) in human. WISP-3 is a member of the CCN (CTGF, Cyr61, NOV) family connective tissue growth modulators involved in cell growth/differentiation during various phases of development through a complex interplay with BMP/Notch/Wnt signaling pathways. WISP-3 is expressed in cells and tissues of mesenchymal origin and WISP3 gene mutations are linked to an autosomal recessive skeletal disorder known as progressive pseudorheumatoid dysplasia (PPRD), indicating its importance in skeletal growth and the maintenance of cartilage integrity. WISP-3 interacts with BMP and components of the Wnt1 signaling pathway including LRP6 and Fz8, promoting the expression of cartilage-specific proteins collagen-II and aggrecan. Both IGF1 and ROS stimulate WISP-3 expression, WISP-3 in turn binds to and blocks IGF1 secretion, preventing IGF1-induced collagen X induction, ROS accumulation, and alkaline phosphatase activity, all of which are associated with chondrocyte hypertrophy induction. Human WISP-3 is synthesized with a signal peptide sequence (a.a. 1-23), the removal of which yields the mature protein containing several protein-interacting domains, including an IGF-interacting IGFBP domain (a.a. 44-117) with a von Willbrand factor type C repeat, a Thrombospondin (TSP) type-1 domain (a.a. 208-253), and a C-terminal cystine knot-like (CTCK) domain (a.a. 268-342).
References
Product Information
FormatPurified
PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect WISP-3 using this mouse monoclonal Anti-WISP-3, clone 13C4.1, Cat. No. MABS1697, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50-1,000 dilution of this antibody detected WISP-3 in human liver, kidney, and lung tissue sections.
Biological Information
ImmunogenGST-tagged recombinant human WISP-3 IGFBP domain fragment.
Clone13C4.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 13C4.1 targets an epitope within the WISP-3 IGFBP domain.
IsotypeIgG1κ
Species Reactivity
  • Rat
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • WISP3
  • CCN6
  • LIBC
  • PPAC
  • PPRD
  • UNQ462/PRO790/PRO956
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~39 kDa observed. 36.84/36.33 kDa (human/rat mature form), 39.29/41.40 kDa (human isoform 1/2 pro-form), and 38.92 kDa (rat pro-form) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected WISP-3 in 10 µg of rat kidney tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1697 04054839055553