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ABE1865 Anti-UTX (KDM6A)

Anti-UTX (KDM6A), Cat. No. ABE1865, is a highly specific rabbit polyclonal antibody that targets Lysine-specific demethylase 6A and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western blotting.

Anti-UTX (KDM6A) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: KDM6A, KABUK2, UTX UniProt Number: O15550
ABE1865
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MChIP, ICC, IF, IP, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE1865
DescriptionAnti-UTX (KDM6A)
Alternate Names
  • Lysine-specific demethylase 6A
  • Histone demethylase UTX
  • Ubiquitously-transcribed TPR protein on the X chromosome
  • Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein
Background InformationLysine-specific demethylase 6A (UniProt O15550; also known as Histone demethylase UTX, KDM6A, Ubiquitously-transcribed TPR protein on the X chromosome, Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein) is encoded by the KDM6A (also known as KABUK2, UTX) gene (Gene ID 7403) in human KDM6A associates with H3K4 methyltransferase MLL3/4 complex and specifically demethylates Lys-27 of histone H3 and plays a central role in histone code. It is shown to demethylate trimethylated and dimethylated, but not monomethylated H3 Lys-27. KDM6A mediates muscle regeneration through it demethylase activity, while it regulates mdsoderm differentiation of embryonic stem cells and mammary gland development independent of its demethylase activity. KDM6A also plays a potential role in regulating brown adipocyte thermogenic program. It is up-regulated during brown adipocyte differentiation and by cold exposure in both brown and white adipose tissue in mice, indicating its potential role in thermogenesis. Inactivation of KDM6A down-regulates brown fat specific gene expression. Mutations in KDM6A gene are a causative factor in Kabuki syndrome that is characterized by congenital mental retardation, postnatal dwarfism, large prominent earlobes, a cleft or high-arched palate, scoliosis, and other abnormalities.

Ref.:
Lederer, D et al. (2012). Am. J. Hum. Genet. 90, 119-124.
Zha, L et al. (2015). J. Biol. Chem. 290, 25151-25163.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-UTX (KDM6A), Cat. No. ABE1865, is a highly specific rabbit polyclonal antibody that targets Lysine-specific demethylase 6A and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western blotting.
Key Applications
  • Chromatin Immunoprecipitation (ChIP)
  • Immunocytochemistry
  • Immunofluorescence
  • Immunoprecipitation
  • Western Blotting
Application NotesChromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected UTX (KDM6A) recruiment to the Myog, CKm, and Tnnc2 promoters following myogenic differentiation of primary murine myoblasts from wild-type or Utx(KI/KI) female mice harboring homozygous demethylase dead Utx mutant gene knock-in (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected a time-dependent UTX (KDM6A) enrichement at the Myogenin and CKm regulatory regions following myogenic differentiation of murine C2C12 myoblasts. shRNA-mediated Six4 knockdown, but not p38 MAPK inhibition, downregulated UTX (KDM6A) recruitment to the Myogenin and CKm loci (Seenundun, S., et al. (2010). EMBO J. 29(8):1401-1411).

Immunocytochemistry Analysis: A representative lot detected UTX (KDM6A) immunoreactivity by indirect immunofluorescence staining of 1% paraformaldehyde-fixed, 0.5% Triton X-100-permeabilized myofibers isolated from mouse extensor digitorum longus (EDL) muscles (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).

Immunofluorescence Analysis: A representative lot detected UTX (KDM6A) immunoreactivity by indirect immunofluorescence staining of 4% paraformaldehyde-fixed, 1% Triton X-100-permeabilized, transverse OCT cryosections sections of mouse tibialis anterior skeletal muscle (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).

Immunoprecipitation Analysis: A representative lot co-immunoprecipitated PTIP and RbBP5 with UTX using embryonic stem cell (ESC) nuclear extracts from wild-type or Utx(KI/KI) mice harboring homozygous demethylase dead Utx mutant gene knock-in. No UTX/PTIP/RbBP5 complex was precipitated from Utx-knockout ESCs (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).

Immunoprecipitation Analysis: A representative lot co-immunoprecipitated Six4 with UTX (KDM6A) using nuclear extract from murine C2C12 myoblasts 24 hrs post myogenic differentiation (Seenundun, S., et al. (2010). EMBO J. 29(8):1401-1411).

Western Blotting Analysis: A representative lot detected UTX4 in myoblast whole cell lysates and embryonic stem cell (ESC) nuclear extracts from wild-type or Utx(KI/KI) mice harboring homozygous demethylase dead Utx mutant gene knock-in, as well as in UTX and MLL4 immunoprecipitates. No UTX target band was detected in Utx-knockout ESCs (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).

Western Blotting Analysis: A representative lot detected UTX (KDM6A) in nuclear extracts from human K562 and murine C2C12 myoblasts (24 hrs post myogenic differentiation), as well as in Six4 immunoprecipitates. shRNA treatment prior to nuclear extract preparation diminished target band detection (Seenundun, S., et al. (2010). EMBO J. 29(8):1401-1411).
Biological Information
ImmunogenHis-tagged recombinant fragment within the central region of human UTX (Seenundun, S., et al. (2010). EMBO J. 29(8):1401-1411).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody detected the expected UTX target band in wild-type, but not Utx-knockout murine ESC nuclear extract (Faralli, H., et al. (2016). J. Clin. Invest. 126(4):1555-1565).
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • KDM6A
  • KABUK2
  • UTX
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~154 kDa observed. 154.2 kDa (human; UniProt O15550) and 154.4/157.5 kDa (mouse isoform 1/2; UniProt O70546) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected UTX (KDM6A) in 10 µg of HeLa nuclear extract.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE1865 04054839132988