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MABN48 Anti-UCHL1 Antibody, clone MCA-BH7

MABN48
100 µL  
Purchase on Sigma-Aldrich

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Overview

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
B, H, RWB, IFMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN48
DescriptionAnti-UCHL1 Antibody, clone MCA-BH7
Alternate Names
  • Ubiquitin carboxyl-terminal hydrolase isozyme L1
  • UCH-L1
  • Neuron cytoplasmic protein 9.5
  • PGP 9.5
  • PGP9.5
  • Ubiquitin thioesterase L1
Background InformationUbiquitin carboxyl-terminal hydrolase isozyme L1 (UCH-L1), a thiol protease belonging to the peptidase C12 family, is involved in the processing of ubiquitin precursors and of ubiquitinated proteins. UCH-L1 recognizes and hydrolyzes peptide bonds at the C-termial glycine of ubiquitin. UCH-L1 is expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Defects in UCH-L1 are the cause of Parkinson disease type 5.
Product Information
FormatPurified
Control
  • Rat brain spinal cord tissue lysate.
PresentationPurified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
Quality LevelMQ100
Applications
ApplicationDetect UCH-L1 using this mouse monoclonal antibody, Anti-UCHL1 Antibody, clone MCA-BH7 validated for use in western blotting & Immunofluorescence.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesWestern Blotting Analysis: A 1:2,000 dilution from a representative lot detected UCHL1 in 10 µg of human brain tissue lysate.

Immunofluorescence Analysis: A 1:2,000 dilution from a representative lot detected UCHL1 in rat brain spinal cord tissue (Gerry Shaw. EnCor Biotechnology, Inc., Gainesville, Florida).
Biological Information
ImmunogenHis-tagged recombinant protein corresponding to human UCHL1.
CloneMCA-BH7
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Bovine
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • UCHL1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~25 kDa observed
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in rat brain spinal cord tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected UCHL1 in 10 µg of rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Global Trade Item Number
Catalogue Number GTIN
MABN48 04053252862953