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48-602MAG
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ST1070
Sigma-AldrichAnti-Tiam1 Rabbit pAb
This Anti-Tiam1 Rabbit pAb is validated for use in Immunoblotting for the detection of Tiam1.
More>>This Anti-Tiam1 Rabbit pAb is validated for use in Immunoblotting for the detection of Tiam1. Less<<
Anti-Tiam1 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Recognizes the ~200 kDa Tiam1 protein in Jurkat and NIH3T3 cells.
Catalogue Number
ST1070
Brand Family
Calbiochem®
Synonyms
Anti-T-Cell Lymphoma Invasion and Metastasis 1
Application Data
Detection of mouse and human Tiam1 by immunoblotting. Samples: whole cell lysate from HIH3T3 cells (50 µg, lane 1) and Jurkat cells (25 µg, lane 2). Primary antibody: Anti-Tiam1 Rabbit pAb (Cat. No. ST1070) (1:1000). Detection: chemiluminescence.
References
References
Mertens, A.E., et al. 2003. FEBS Lett.546, 11. Qi, H., et al. 2001. Cell Growth Differ.12, 603. Stam, J.C., et al. 1997. J. Biol. Chem.272, 28447.
Mertens, A.E., et al. 2003. FEBS Lett.546, 11. Qi, H., et al. 2001. Cell Growth Differ.12, 603. Stam, J.C., et al. 1997. J. Biol. Chem.272, 28447.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
18-October-2007 RFH
Synonyms
Anti-T-Cell Lymphoma Invasion and Metastasis 1
Application
Immunoblotting (1:1000)
Application Data
Detection of mouse and human Tiam1 by immunoblotting. Samples: whole cell lysate from HIH3T3 cells (50 µg, lane 1) and Jurkat cells (25 µg, lane 2). Primary antibody: Anti-Tiam1 Rabbit pAb (Cat. No. ST1070) (1:1000). Detection: chemiluminescence.
Tiam1 (T-cell lymphoma invasion and metastasis 1) was originally identified by its ability to induce invasion by T-lymphoma cells when amplified. Tiam1 is a specific guanine nucleotide exchange factor (GEF) for the GTPase, Rac. By activating Rac1, Tiam1 regulates many cellular functions including actin cytoskeleton dynamics, cell proliferation, and cell survival. Tiam1 activity is regulated by its translocation to the membrane and the subsequent binding of its PH domain to phosphatidylinositol-3,4,5-triphosphate. Tiam1 is cleaved in a caspase-dependent manner in apoptotic cells, rendering it inactive as a result of an inability to translocate to the plasma membrane.
Host
Rabbit
Immunogen species
Human
Immunogen
a synthetic peptide corresponding to amino acids from the C-terminal domain of human Tiam1
Isotype
IgG
Species
human, mouse
Positive control
Jurkat or NIH3T3 cells
Form
Liquid
Formulation
In Tris-Citrate/phosphate, pH 7.0-8.0.
Concentration Label
Please refer to vial label for lot-specific concentration
Preservative
≤0.1% sodium azide
Comments
Antibody should be titrated for optimal results in individual systems.
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
Toxicity
Highly Toxic
References
Mertens, A.E., et al. 2003. FEBS Lett.546, 11. Qi, H., et al. 2001. Cell Growth Differ.12, 603. Stam, J.C., et al. 1997. J. Biol. Chem.272, 28447.