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PC392 Anti-TRAIL Receptor-2 (Ab-1) Rabbit pAb

Anti-TRAIL Receptor-2 (Ab-1) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Death Receptor, Anti-APO2, Anti-DR5

PC392
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes ~55-58 kDa TRAIL receptor-2 (DR5) in HeLa and K562 cells.
Catalogue NumberPC392
Brand Family Calbiochem®
SynonymsAnti-Death Receptor, Anti-APO2, Anti-DR5
Application Data
Detection of human TRAIL receptor 2 by immunoblotting. Sample: Whole cell lysate (50 μg) from HeLa and K562 cells. Primary antibody: Anti-TRAIL Receptor-2 (DR5) (Ab-1) Rabbit pAb (Cat. No. PC392) (1:500). Detection: chemiluminescence.

Detection of human TRAIL receptor-2 by immunocytochemistry. Sample: Formalin-fixed HeLa cells. Primary antibody: Anti-TRAIL Receptor-2 (Ab-1) Rabbit pAb (Cat. No. PC392) (5 µg/ml). Detection: chemiluminescence.
References
ReferencesChaudhary, P.M., et al. 1997. Immunity 7, 821.
MacFarlane, M., et al. 1997. J. Biol. Chem. 272, 25417.
Pan, G., et al. 1997. Science 277, 815.
Screaton, G.R., et al. 1997. Curr. Biol. 7, 693.
Sheridan, J.P., et al. 1997. Science 277, 818.
Walczak, H., et al. 1997. EMBO. J. 16, 5386.
Wu, G.S., et al. 1997. Nat. Genet. 17, 141.
Product Information
FormLiquid
FormulationIn PBS, 0.05% BSA.
Positive controlHeLa cells
Preservative≤0.1% sodium azide
Applications
Application ReferencesParaffin Sections Spierings DCJ, et al. 2003. Clin Cancer Res. 9,3397.
Key Applications Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesImmunoblotting (1:500-1:1000)
Paraffin Sections (5 µg/ml, see application references)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (KWVNKTGRDASVHTLLDALE) corresponding to amino acids 388-407 of human TRAIL receptor-2 (DR5) precursor
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Highly Toxic
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC392 0

Documentation

Anti-TRAIL Receptor-2 (Ab-1) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-TRAIL Receptor-2 (Ab-1) Rabbit pAb Certificates of Analysis

TitleLot Number
PC392

References

Reference overview
Chaudhary, P.M., et al. 1997. Immunity 7, 821.
MacFarlane, M., et al. 1997. J. Biol. Chem. 272, 25417.
Pan, G., et al. 1997. Science 277, 815.
Screaton, G.R., et al. 1997. Curr. Biol. 7, 693.
Sheridan, J.P., et al. 1997. Science 277, 818.
Walczak, H., et al. 1997. EMBO. J. 16, 5386.
Wu, G.S., et al. 1997. Nat. Genet. 17, 141.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision30-April-2008 JSW
SynonymsAnti-Death Receptor, Anti-APO2, Anti-DR5
ApplicationImmunoblotting (1:500-1:1000)
Paraffin Sections (5 µg/ml, see application references)
Application Data
Detection of human TRAIL receptor 2 by immunoblotting. Sample: Whole cell lysate (50 μg) from HeLa and K562 cells. Primary antibody: Anti-TRAIL Receptor-2 (DR5) (Ab-1) Rabbit pAb (Cat. No. PC392) (1:500). Detection: chemiluminescence.

Detection of human TRAIL receptor-2 by immunocytochemistry. Sample: Formalin-fixed HeLa cells. Primary antibody: Anti-TRAIL Receptor-2 (Ab-1) Rabbit pAb (Cat. No. PC392) (5 µg/ml). Detection: chemiluminescence.
DescriptionProtein G purified rabbit polyclonal antibody. Recognizes the ~55-58 kDa TRAIL receptor-2 protein.
BackgroundApoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors. TRAIL/Apo2L is a new member of the TNF family. DR4 was recently identified as the receptor for TRAIL. A novel death domain containing receptor for TRAIL was more recently identified and designated DR5, Apo2, TRAIL-R2, TRICK2, or KILLER by several groups independently. Like DR4, DR5 transcript is widely expressed in normal tissues and in many types of tumor cells. DR5 binds to TRAIL and mediates TRAIL induced cell death. Overexpression of DR5 induces apoptosis and activates NF-κB.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide (KWVNKTGRDASVHTLLDALE) corresponding to amino acids 388-407 of human TRAIL receptor-2 (DR5) precursor
IsotypeIgG
Specieshuman
Positive controlHeLa cells
FormLiquid
FormulationIn PBS, 0.05% BSA.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Highly Toxic
ReferencesChaudhary, P.M., et al. 1997. Immunity 7, 821.
MacFarlane, M., et al. 1997. J. Biol. Chem. 272, 25417.
Pan, G., et al. 1997. Science 277, 815.
Screaton, G.R., et al. 1997. Curr. Biol. 7, 693.
Sheridan, J.P., et al. 1997. Science 277, 818.
Walczak, H., et al. 1997. EMBO. J. 16, 5386.
Wu, G.S., et al. 1997. Nat. Genet. 17, 141.
Application referencesParaffin Sections Spierings DCJ, et al. 2003. Clin Cancer Res. 9,3397.