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MABC1142-25UL Anti-TIMP-4 Antibody, clone 9:4-7

Anti-TIMP-4, clone 9:4-7, Cat. No. MABC1142, is a mouse monoclonal antibody that detects Tissue Inhibitor of Matrix Metalloproteinase 4 and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

MABC1142-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABC1142-25UL
DescriptionAnti-TIMP-4 Antibody, clone 9:4-7
Alternate Names
  • Tissue inhibitor of metalloproteinases 4
  • Metalloproteinase inhibitor 4
Background InformationTissue inhibitor of metalloproteinases 4 (UniProt: Q99727; also known as Metalloproteinase inhibitor 4, TIMP-4) is encoded by the TIMP4 gene (Gene ID: 7079) in human. The tissue inhibitors of metalloproteinases (TIMPs) represent a family of ubiquitous proteins which are natural inhibitors of the matrix metalloproteinases (MMPs). There are four known members of the TIMP family that are designated as TIMP-1, 2, 3, and 4. TIMP-4 is synthesized with a signal peptide (aa 1-29), which is cleaved off in the mature form. It has two MMP-binding regions (aa 30-33 and 99-100). TIMP-4 expressed is observed in heart, brain, pancreas, kidney, colon, testes, and adipose tissue. However, elevated levels of TIMP-4 have been reported in various cancers tissues, including breast, ovary, prostate, colon and kidney and are associated with poor survival prognosis. Hence, it can serve as a useful marker for disease progression. TIMP-4 is shown to complex MMPs and irreversibly inactivates them by binding to their catalytic zinc cofactor. This clone (9:4 7) is specific for human TIMP-4 and does not exhibit any cross-reactivity to other TIMPs. (Ref.: Gomez, DE., et al. (1997). Eur. J. Cell. Biol. 74(2); 111-122; Donover, PS et AL (2010). J. Cell. Biochem. 110(5); 1255-1261).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-TIMP-4, clone 9:4-7, Cat. No. MABC1142, is a mouse monoclonal antibody that detects Tissue Inhibitor of Matrix Metalloproteinase 4 and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • ELISA
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected TIMP-4 in human kidney tissue sections.

Immunohistochemistry (Paraffin) Analysis: A representative lot detected TIMP-4 in Immunohistochemistry applications (Donover, P.S., et. al. (2010). J Cell Biochem. 110(5):1255-61).

ELISA Analysis: A representative lot detected TIMP-4 in ELISA applications (Donover, P.S., et. al. (2010). J Cell Biochem. 110(5):1255-61).

Western Blotting Analysis: A representative lot detected TIMP-4 in Western Blotting applications (Donover, P.S., et. al. (2010). J Cell Biochem. 110(5):1255-61).
Biological Information
ImmunogenGST-tagged full-length recombinant human TIMP-4.
Clone9:4-7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 9:4-7 specifically detects human TIMP-4.
IsotypeIgG2bκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • TIMP4
Purification MethodProtein G purified
UniProt Number
Molecular Weight25.50 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human breast cancer tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected TIMP-4 in human breast cancer tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1142-25UL 04054839659997