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MABF2657-25UL Anti-TIGIT Antibody, clone 1G9

Anti-TIGIT, clone 1G9, Cat. No. MABF2657, is a mouse monoclonal antibody that detects of T-cell immunoreceptor with Ig and ITIM domains (TIGIT) and is tested in ELISA, Flow Cytometry, Inhibition Assay, and Western Blotting.

MABF2657-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2657-25UL
DescriptionAnti-TIGIT Antibody, clone 1G9
Alternate Names
  • T-cell immunoreceptor with Ig and ITIM domains
  • V-set and transmembrane domain-containing protein 3
Background InformationT-cell immunoreceptor with Ig and ITIM domains (UniProt: P86176; also known as V-set and immunoglobulin domain-containing protein 9, TIGIT) is encoded by the Tigit (also known as Vstm3) gene (Gene ID: 100043314) in murine species. TIGIT is a single-pass type I, homodimeric, membrane protein that is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 29-148), a transmembrane domain (aa 149-169), and a cytoplasmic domain (aa 170-249). The cytoplasmic domain contains an immunoreceptor tyrosine-based inhibitor motif (ITIM; aa 234-239), which is involved in the modulation of its cellular responses. It serves as a coinhibitory receptors with CD226 and is expressed on NK cells, activated T cells, memory T cells, and a subset of regulatory T cells and blocks immune responses by affecting T cells and APCs. It is shown to bind to poliovirus receptor (PVR) with high affinity to form a heterotetrameric assembly of two TIGIT and two PVR molecules. This causes increased secretion of IL-10 and decreased secretion of IL-12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells. TIGIT also serves as a functional ligand and induces dendritic cells to acquire a tolerogenic phenotype via ligation of PVR. TIGIT -/- mice, when challenged, develop severe experimental autoimmune encephalomyelitis compared to wild-type mice. Loss of TIGIT also in mice also results in hyperproliferative T cell responses and increased susceptibility to autoimmunity. (Ref.: Dixon, KO., et al. (2018). J. Immunol. 200(8); 3000-3007; Joller, N., et al. (2011). J. Immunol. 186(3); 1338-1342).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-TIGIT, clone 1G9, Cat. No. MABF2657, is a mouse monoclonal antibody that detects of T-cell immunoreceptor with Ig and ITIM domains (TIGIT) and is tested in ELISA, Flow Cytometry, Inhibition Assay, and Western Blotting.
Key Applications
  • Western Blotting
  • Flow Cytometry
  • ELISA
  • Agonist or Inhibitor
Application NotesTested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TIGIT in mouse thymus tissue lysate.

ELISA Analysis: A representative lot detected TIGIT in ELISA applications (Joller, N., et al. (2011). J Immunol. 186(3):1388-42).

Flow Cytometry Analysis: A representative lot detected TIGIT in Flow Cytometry applications (Joller, N., et al. (2011). J Immunol. 186(3):1388-42).

Inhibition Assay: A representative lot of this antibody fully blocked CD155 binding to TIGIT. (Dixon, K.O., et al. (2018). J Immunol. 200(8):3000-3007).

Flow Cytometry Analysis: 1 µg from a representative lot detected TIGIT in one million Raw264.7 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenTIGIT knockout mice immunized with recombinant mouse TIGIT tetramers.
EpitopeUnknown
Clone1G9
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1G9 is a mouse monoclonal antibody that detects murine T-cell immunoreceptor with Ig and ITIM domains (TIGIT).
IsotypeIgG1
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Gene Symbol
  • Tigit
Purification MethodProtein G purified
UniProt Number
Molecular Weight~31 kDa observed; 26.96 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected T-cell immunoreceptor with Ig and ITIM domains (TIGIT) s in RAW264.7 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF2657-25UL 04065266876338