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GF06 Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3)

This Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3) is validated for use in Immunoblotting for the detection of TGF-α (Ab-1).

Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Transforming Growth Factor-α

GF06
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, RMMonoclonal Antibody
Description
OverviewRecognizes the ~6 kDa TGF-α protein in A2058 and ST-FeSVFRE cells.
Catalogue NumberGF06
Brand Family Calbiochem®
SynonymsAnti-Transforming Growth Factor-α
References
ReferencesSorvillo, J.M., McCormack, E.S. 1990. Oncogene 5, 377.
Coffey, R.J., et al. 1987. Nature 328, 817.
Derynck, R. 1986. J. Cell. Biochem. 32, 203.
Samsoondor, J., et al. 1986. J. Biol. Chem. 261, 14408.
Lee, D.C., et al. 1985 Mol. Cell. Biol. 5, 3644.
Kimball, E.S., et al. 1984. Cancer Res. 44, 3613.
Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4684.
Reynolds, F.H., Jr., et al. 1983. Nature 292, 259.
Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press 165.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Negative controlNIH3T3 cells
Positive controlA2058 or ST-FeSV FRE cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone, Species Reactivity Sorvillo, J.M., McCormack, E.S., 1990. Oncogene 5, 377. Immunoblotting Leong, H., et al. 2001. Carcinogenesis 22, 1809. Bush, M.R., et al. 1998. Biol. Reprod. 59, 1522.
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1-5 µg/ml, see application references)
Application CommentsThis antibody shows no cross-reactivity with EGF. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human TGF-α (~6 kDa mature form)
ImmunogenHuman
Clone134A-2B3
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) or aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF06 0

Documentation

Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3) SDS

Title

Safety Data Sheet (SDS) 

Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3) Certificates of Analysis

TitleLot Number
GF06

References

Reference overview
Sorvillo, J.M., McCormack, E.S. 1990. Oncogene 5, 377.
Coffey, R.J., et al. 1987. Nature 328, 817.
Derynck, R. 1986. J. Cell. Biochem. 32, 203.
Samsoondor, J., et al. 1986. J. Biol. Chem. 261, 14408.
Lee, D.C., et al. 1985 Mol. Cell. Biol. 5, 3644.
Kimball, E.S., et al. 1984. Cancer Res. 44, 3613.
Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4684.
Reynolds, F.H., Jr., et al. 1983. Nature 292, 259.
Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press 165.

Citations

Title
  • Hoyee Leong, Gary L. Firestone and Leonard B. Bjeldanes. (2001) Cytostatic Effects of 3,3'-Diindolylmethane in Human Endometrial Cancer Cells Result From an Estrogen Receptor-Mediated Increase in Transforming Growth Factor-α Expression. Carcinogenesis 22, 1809-1817.
  • Mark R. Bush, et al. (1998) Evidence of Juxtacrine Signaling for Transforming Growth Factor a in Human Endometrium. 59, 1522-1529.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision23-August-2007 RFH
    SynonymsAnti-Transforming Growth Factor-α
    ApplicationImmunoblotting (1-5 µg/ml, see application references)
    DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with P3 x 63 Ag 8.653 mouse myeloma cells (see application references). Recognizes the ~6 kDa denatured and native forms of TGFα protein.
    BackgroundTransforming growth factor-alpha (TGFα) is an acid- and heat-stable 50 amino acid protein of 5500 MW originally found in rodents and humans. TGFαis 33% homologous at the amino acid level to epidermal growth factor (EGF). TGFα binds to the EGF receptor, mediates tyrosine phosphorylation of the receptor, and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor-beta. TGFα is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells. TGFα bioactivity has been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.
    HostMouse
    Immunogen speciesHuman
    Immunogenrecombinant, human TGF-α (~6 kDa mature form)
    Clone134A-2B3
    IsotypeIgG₁
    Specieshuman, rat
    Positive controlA2058 or ST-FeSV FRE cells
    Negative controlNIH3T3 cells
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsThis antibody shows no cross-reactivity with EGF. Antibody should be titrated for optimal results in individual systems.
    Storage +2°C to +8°C
    Do Not Freeze Yes
    Special InstructionsWe recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) or aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesSorvillo, J.M., McCormack, E.S. 1990. Oncogene 5, 377.
    Coffey, R.J., et al. 1987. Nature 328, 817.
    Derynck, R. 1986. J. Cell. Biochem. 32, 203.
    Samsoondor, J., et al. 1986. J. Biol. Chem. 261, 14408.
    Lee, D.C., et al. 1985 Mol. Cell. Biol. 5, 3644.
    Kimball, E.S., et al. 1984. Cancer Res. 44, 3613.
    Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4684.
    Reynolds, F.H., Jr., et al. 1983. Nature 292, 259.
    Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press 165.
    Citation
  • Hoyee Leong, Gary L. Firestone and Leonard B. Bjeldanes. (2001) Cytostatic Effects of 3,3'-Diindolylmethane in Human Endometrial Cancer Cells Result From an Estrogen Receptor-Mediated Increase in Transforming Growth Factor-α Expression. Carcinogenesis 22, 1809-1817.
  • Mark R. Bush, et al. (1998) Evidence of Juxtacrine Signaling for Transforming Growth Factor a in Human Endometrium. 59, 1522-1529.
    		•
    Application referencesOriginal Clone, Species Reactivity Sorvillo, J.M., McCormack, E.S., 1990. Oncogene 5, 377. Immunoblotting Leong, H., et al. 2001. Carcinogenesis 22, 1809. Bush, M.R., et al. 1998. Biol. Reprod. 59, 1522.