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GF16 Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2)

This Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of TGF-β3 (Ab-1).

Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Transforming Growth Factor-β3

GF16
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes TGF-β2 and TGF-β3 in umbilical cord cells. Does not cross-react with TGF-β1.
Catalogue NumberGF16
Brand Family Calbiochem®
SynonymsAnti-Transforming Growth Factor-β3
References
ReferencesBurton, P.B.J., et al. 1993 Int. J. Exp. Path. 74, 87.
Roberts, A.B., et al. 1990 Philos. Trans. R. Soc. Lond. [Biol] 327, 145.
ten Dijke, P., et al. 1990 Mol. Cell. Biol. 10, 4473.
ten Dijke, P., et al. 1988 Proc. Natl. Acad. Sci. USA 85, 4715.
de Martin, R., et al. 1987 EMBO J. 6, 3677.
Sporn, M.B., et al. 1987 J. Cell Biol. 105, 1039.
Derynck, R., et al. 1985 Nature 316, 701.
Anzano, M.A., et al. 1983 Proc. Natl. Acad. Sci. USA 80, 6264.
Todaro, G.J., et al. 1980 Proc. Natl. Acad. Sci. USA 77, 5258.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
Positive controlRecombinant TGF-β3 protein (Cat. No. PF018) or umbilical cord cells
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunoprecipitation
Paraffin Sections
Application NotesFrozen Sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (1-10 µg/ml, no pre-treatment required, see comments)
Application CommentsDoes not cross-react with TGF-β1. For staining paraffin sections, no pre-treatment is required, but the addition of 0.05% Triton® X-100 detergent in the incubation buffer is recommended. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human TGF-β3
ImmunogenHuman
Epitopewithin the N-terminal region
Clone236-5.2
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
GF16 0

Documentation

Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2) SDS

Title

Safety Data Sheet (SDS) 

Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2) Certificates of Analysis

TitleLot Number
GF16

References

Reference overview
Burton, P.B.J., et al. 1993 Int. J. Exp. Path. 74, 87.
Roberts, A.B., et al. 1990 Philos. Trans. R. Soc. Lond. [Biol] 327, 145.
ten Dijke, P., et al. 1990 Mol. Cell. Biol. 10, 4473.
ten Dijke, P., et al. 1988 Proc. Natl. Acad. Sci. USA 85, 4715.
de Martin, R., et al. 1987 EMBO J. 6, 3677.
Sporn, M.B., et al. 1987 J. Cell Biol. 105, 1039.
Derynck, R., et al. 1985 Nature 316, 701.
Anzano, M.A., et al. 1983 Proc. Natl. Acad. Sci. USA 80, 6264.
Todaro, G.J., et al. 1980 Proc. Natl. Acad. Sci. USA 77, 5258.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision23-August-2007 RFH
SynonymsAnti-Transforming Growth Factor-β3
ApplicationFrozen Sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (1-10 µg/ml, no pre-treatment required, see comments)
DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3x63 Ag 8.653 myeloma cells. Recognizes TGF-β2 and TGF-β3.
BackgroundTransforming growth factorβs are a family of proteins originally characterized by their ability to act synergistically with EGF or TGFα. TGFβs have been found to produce multiple biological effects including mitogenesis, growth inhibition, chemotaxis and induction or inhibition of differentiation. In their mature form TGFβs are acid- and heat-stable disulfide-linked homodimeric proteins (Mr = ~25 kDa), with each homodimer consisting of 112 amino acid residues. Two homodimeric forms of TGFβ have been identified (TGFβ1 and TGFβ2) which show significant amino acid sequence homology. A new member of the family, TGFβ3 has recently been cloned and characterized. There is greater amino acid homology between TGFβ3 and TGFβ2 as compared with TGFβ1. All TGFβs are released by cells in a biologically latent form. Most cells have high affinity receptors for TGFβ1, β2, and β3.
HostMouse
Immunogen speciesHuman
Immunogenrecombinant, human TGF-β3
Epitopewithin the N-terminal region
Clone236-5.2
IsotypeIgG₁
Specieshuman, mouse, rat
Positive controlRecombinant TGF-β3 protein (Cat. No. PF018) or umbilical cord cells
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
Concentration Label Please refer to vial label for lot-specific concentration
PreservativeNone
CommentsDoes not cross-react with TGF-β1. For staining paraffin sections, no pre-treatment is required, but the addition of 0.05% Triton® X-100 detergent in the incubation buffer is recommended. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesBurton, P.B.J., et al. 1993 Int. J. Exp. Path. 74, 87.
Roberts, A.B., et al. 1990 Philos. Trans. R. Soc. Lond. [Biol] 327, 145.
ten Dijke, P., et al. 1990 Mol. Cell. Biol. 10, 4473.
ten Dijke, P., et al. 1988 Proc. Natl. Acad. Sci. USA 85, 4715.
de Martin, R., et al. 1987 EMBO J. 6, 3677.
Sporn, M.B., et al. 1987 J. Cell Biol. 105, 1039.
Derynck, R., et al. 1985 Nature 316, 701.
Anzano, M.A., et al. 1983 Proc. Natl. Acad. Sci. USA 80, 6264.
Todaro, G.J., et al. 1980 Proc. Natl. Acad. Sci. USA 77, 5258.