Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

ABE598 Anti-TCF12 Antibody

Detect TCF12 using this rabbit polyclonal antibody, Anti-TCF12 Antibody validated for use in western blotting, ICC & IHC.

Anti-TCF12 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: TCF12 UniProt Number: Q99081
ABE598
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICC, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABE598
DescriptionAnti-TCF12 Antibody
Alternate Names
  • HTF4
  • HEB
  • BHLHB20
  • Transcription factor 12
  • TCF-12
  • Class B basic helix-loop-helix protein 20
  • bHLHb20
  • DNA-binding protein HTF4
  • E-box-binding protein
  • Transcription factor HTF-4
Background InformationTCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family. TCF12 is a transcriptional regulator involved in the initiation of neuronal differentiation, and it has been shown to activate transcription by binding to the E box (5'-CANNTG-3'). TCF12 has been shown to interact with PTF1A, NEUROD2 and RUNX1T1. TCF12 is suggested to play a critical role in proper T cell differentiation by blocking the thymocyte proliferation in combination with E2A. Recent studies have shown that TCF12 is also an important factor required for the development of invariant natural killer T cells. TCF12 is expressed in many tissues, and more specifically in skeletal muscle, thymus, B- and T-cells.
References
Product Information
FormatAffinity Purified
Control
  • HeLa cell lysate
PresentationPurified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect TCF12 using this rabbit polyclonal antibody, Anti-TCF12 Antibody validated for use in western blotting, ICC & IHC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected TCF12 in human tonsil tissue.

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected TCF12 in HeLa cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to human TCF12.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Gene Symbol
  • TCF12
Purification MethodAntigen Affinity Purified
UniProt Number
Molecular Weight~83 kDa observed. Uncharacterized bands may appear in some lysates. Uniprot describes 3 isoforms between ~55 kDa and ~76 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TCF12 in 15 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ABE598 04053252923418