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PC325 Anti-α-Synuclein (Ab-1) (123-140) Guinea pig pAb

This Anti-α-Synuclein (Ab-1) (123-140) Guinea pig pAb is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence for the detection of α-Synuclein (Ab-1) (123-140).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
PC325
  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, H, M, RGpPolyclonal Antibody
Description
Overview

This product has been discontinued.




Recognizes the α-synuclein protein.
Catalogue NumberPC325
Brand Family Calbiochem®
References
ReferencesTakeda, A., et al. 1998. Am. J. Pathol. 152, 367.
Nussbaum, R.L. and Polymeropoulos, M.H., 1997. Hum. Mol. Genet. 6, 1687.
Wakabayashi, K., et al. 1997. Neurosci. Lett. 239, 45.
Campion, D., et al. 1995. Genomics 26, 254.
Iwai, A., et al. 1995. Neuron 14, 467.
Brookes, A.J. and St. Clair, D. 1994. Trends Neurosci. 17, 404.
Product Information
FormLiquid
FormulationUndiluted serum.
Preservative≤0.1% sodium azide
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Application NotesFrozen Sections (1:8000-1:10,000)
Immunoblotting (1:2000, chemiluminescence)
Immunofluorescence (1:8000-1:10,000)
Application CommentsCan be used to localize α-synuclein in the central nervous system. For frozen sections, tissue should be fixed in 4% paraformaldehyde and 0.4% picric acid in 0.16 M sodium phosphate buffer, pH 6.9. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (EAYEMPSEEGYQDYEPEA) corresponding to amino acids 123-140 of the C-terminus of rat α-synuclein
ImmunogenRat
HostGuinea Pig
IsotypeIgG
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
PC325 0

Documentation

Anti-α-Synuclein (Ab-1) (123-140) Guinea pig pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-α-Synuclein (Ab-1) (123-140) Guinea pig pAb Certificates of Analysis

TitleLot Number
PC325

References

Reference overview
Takeda, A., et al. 1998. Am. J. Pathol. 152, 367.
Nussbaum, R.L. and Polymeropoulos, M.H., 1997. Hum. Mol. Genet. 6, 1687.
Wakabayashi, K., et al. 1997. Neurosci. Lett. 239, 45.
Campion, D., et al. 1995. Genomics 26, 254.
Iwai, A., et al. 1995. Neuron 14, 467.
Brookes, A.J. and St. Clair, D. 1994. Trends Neurosci. 17, 404.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-October-2007 RFH
ApplicationFrozen Sections (1:8000-1:10,000)
Immunoblotting (1:2000, chemiluminescence)
Immunofluorescence (1:8000-1:10,000)
DescriptionGuinea pig polyclonal antibody supplied as undiluted serum. Recognizes the α-synuclein protein.
BackgroundThe major component of the vascular and plaque amyloid deposits in Alzheimer's disease (AD) is the amyloid β peptide. Non-A β component of Alzheimer's disease amyloid (NAC) is the second component in the amyloid plaques from brain tissues of AD patients. Its precursor protein (NACP) is now known to be identical to α-synuclein, a presynaptic protein in the human brain. In AD, synuclein/NAC proteins are found in presynaptic cholinergic nerve terminals that degenerate early in AD and are closely linked to β amyloid fibrils in senile plaques. α-synuclein is a highly conserved protein that shows 95% homology with rat synuclein 1 and good homology with Torpedo synuclein from the electric organ synapse and bovine phosphoneuroprotein 14 (PNP-14), a brain specific protein present in synapses. Recently α-synuclein has been linked to some cases of familial Parkinson's disease (PD), localizing in Lewy bodies, hallmark lesions of degenerating neurons in the brains of patients with PD. A missense mutation in the gene encoding α-synuclein gene has been identified in some of these PD patients.
HostGuinea Pig
Immunogen speciesRat
Immunogena synthetic peptide (EAYEMPSEEGYQDYEPEA) corresponding to amino acids 123-140 of the C-terminus of rat α-synuclein
IsotypeIgG
Speciesbovine, human, mouse, rat
FormLiquid
FormulationUndiluted serum.
Preservative≤0.1% sodium azide
CommentsCan be used to localize α-synuclein in the central nervous system. For frozen sections, tissue should be fixed in 4% paraformaldehyde and 0.4% picric acid in 0.16 M sodium phosphate buffer, pH 6.9. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesTakeda, A., et al. 1998. Am. J. Pathol. 152, 367.
Nussbaum, R.L. and Polymeropoulos, M.H., 1997. Hum. Mol. Genet. 6, 1687.
Wakabayashi, K., et al. 1997. Neurosci. Lett. 239, 45.
Campion, D., et al. 1995. Genomics 26, 254.
Iwai, A., et al. 1995. Neuron 14, 467.
Brookes, A.J. and St. Clair, D. 1994. Trends Neurosci. 17, 404.