Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

DP01 Anti-SV40 T Antigen (Ab-1) Mouse mAb (PAb419)

This Anti-SV40 T Antigen (Ab-1) Mouse mAb (PAb419) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of SV40 T Antigen (Ab-1).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
DP01
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Sv40 Infected CellsMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
DP01-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the ~94 kDa SV40 large T antigen and the ~21 kDa SV40 small T antigen.
Catalogue NumberDP01
Brand Family Calbiochem®
References
ReferencesDeCaprio, J.A., et al.1988. Cell 54, 275.
Whyte, P., et al. 1988 Nature 334, 124.
Mitchell, P.J., et al.1987 Cell 50, 847.
Dixon, R.A.F. and Nathans, D., 1985 J. Virol. 53, 1001.
Simanis, V. and Lane, D.P., 1985 Virol. 144, 88.
Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
Tooze, J.1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol. 10, 591.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Positive controlSV80 or SV-T2 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone Harlow, E., et al. 1981 J. Virol. 39, 861. Immunoblotting Goolsby, C.L., et al. 1991. Cytometry 12, 748.
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesImmunoblotting (see application references)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenthe N-terminal domain of SV40 large and small T-antigen
ClonePAb419
HostMouse
IsotypeIgG2a
Species Reactivity
  • Sv40 Infected Cells
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
DP01-100UG 04055977226546

Documentation

Anti-SV40 T Antigen (Ab-1) Mouse mAb (PAb419) Certificates of Analysis

TitleLot Number
DP01

References

Reference overview
DeCaprio, J.A., et al.1988. Cell 54, 275.
Whyte, P., et al. 1988 Nature 334, 124.
Mitchell, P.J., et al.1987 Cell 50, 847.
Dixon, R.A.F. and Nathans, D., 1985 J. Virol. 53, 1001.
Simanis, V. and Lane, D.P., 1985 Virol. 144, 88.
Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
Tooze, J.1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol. 10, 591.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-August-2007 RFH
ApplicationImmunoblotting (see application references)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~94 kDa SV40 large T antigen and the ~20.5 kDa SV40 small T antigen.
BackgroundSV40 T Ag (Ab-1) is a mouse monoclonal antibody with specificity for antigenic determinants localized to the amino terminal domain of the SV40 large T antigen. The sequences recognized by this reagent are also present on SV40 small T antigen. Both antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the P105 product of the retinoblastoma susceptibility gene.
HostMouse
Immunogenthe N-terminal domain of SV40 large and small T-antigen
ClonePAb419
IsotypeIgG2a
SpeciesSV40 infected cells
Positive controlSV80 or SV-T2 cells
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesDeCaprio, J.A., et al.1988. Cell 54, 275.
Whyte, P., et al. 1988 Nature 334, 124.
Mitchell, P.J., et al.1987 Cell 50, 847.
Dixon, R.A.F. and Nathans, D., 1985 J. Virol. 53, 1001.
Simanis, V. and Lane, D.P., 1985 Virol. 144, 88.
Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
Tooze, J.1973. Cold Spring Harbor, New York.
Tegtmeyer, P. 1972. J. Virol. 10, 591.
Application referencesOriginal Clone Harlow, E., et al. 1981 J. Virol. 39, 861. Immunoblotting Goolsby, C.L., et al. 1991. Cytometry 12, 748.