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MABT521 Anti-SMAD3, clone 5G11 Antibody

Anti-SMAD3, clone 5G11 Antibody is an antibody against SMAD3 for use in Western Blotting and Immunocytochemistry.

Anti-SMAD3, clone 5G11 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: SMAD3, MADH3 UniProt Number: P84022
MABT521
100 µL  
Purchase on Sigma-Aldrich

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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ICCMAscitesMonoclonal Antibody
Description
Catalogue NumberMABT521
Replaces04-1035
DescriptionAnti-SMAD3, clone 5G11 Antibody
Alternate Names
  • Mothers against decapentaplegic homolog 3
  • MAD homolog 3
  • Mad3
  • Mothers against DPP homolog 3
  • hMAD-3
  • JV15-2
  • SMAD family member 3
  • SMAD 3
  • Smad3
  • hSMAD3
  • SMAD3
Background InformationMembers of the SMAD family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-β signals from the cell surface into the nucleus. Three distinct classes of SMADs have been defined: the receptor-regulated SMADs (R-SMADs), which include Smad1, 2, 3, 5 and 8, the common-mediator SMAD (co-SMAD), SMAD4, and the antagonistic or inhibitory SMADs (I-SMADs), SMAD6 and 7. Once in the nucleus, SMADs can target a variety of DNA binding proteins to regulate transcriptional responses. Following stimulation by TGF-β, SMAD2 and SMAD3 become phosphorylated at their carboxyl termini (Ser465 and 467 on SMAD2; Ser423 and 425 on SMAD3) by TGF-β Receptor I. Phosphorylated SMAD 2/3 can complex with SMAD4, translocate to the nucleus and regulate gene expression.
References
Product Information
FormatAscites
PresentationMouse monoclonal IgG1ascitic fluid containing 0.03% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-SMAD3, clone 5G11 Antibody is an antibody against SMAD3 for use in Western Blotting and Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesImmunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected SMAD3 in NIH/3T3 cells.
Biological Information
ImmunogenPurified recombinant fragment of human SMAD3 expressed in E. Coli.
Clone5G11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Gene Symbol
  • SMAD3
  • MADH3
Purification MethodUnpurified
UniProt Number
Molecular Weight~48 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected SMAD3 in A549 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABT521 04055977171518