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MABF3047-100UG Anti-SCARB1 Antibody, clone AOB-1

Anti-SCARB1, clone AOB-1, Cat. No. MABF3047, is a rat monoclonal antibody that detects SCARB1 and is tested for use in Flow Cytometry, Immunohistochemistry, Inhibition Assay, and Western Blotting.

MABF3047-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF3047-100UG
DescriptionAnti-SCARB1 Antibody, clone AOB-1
Alternate Names
  • Scavenger receptor class B member 1
  • SRB1
  • SR-BI
Background InformationScavenger receptor class B member 1 (UniProt: Q61009; also known as SRB1, SR-BI) is encoded by the Scarb1 (also known as Srb1) gene (Gene ID: 20778) in murine species. SR-BI (SCARB1) is a widely expressed, multi-pass membrane glycoprotein with two cytoplasmic domains (aa 1-11 and 462-509), one extracellular domain (aa 33-440) and two transmembrane domains (aa 12-32 and 441-461). It serves as a receptor for several different ligands such as phospholipids, cholesterol ester, lipoproteins, and phosphatidylserine. It is a key component in the reverse cholesterol transport pathway and thus plays an important role in lipid metabolism. It may also contribute to variations in plasma lipid levels during fasting. Two isoforms of SR-BI have been described that are produced by alternative splicing and both isoforms can serve as a receptor for HDL and mediate selective uptake of cholesteryl ether and HDL-dependent cholesterol efflux. It is reported to facilitate the flux of free and esterified cholesterol between the cell surface and ApoB-containing lipoproteins and modified lipoproteins, although less efficiently than HDL. SR-BI is also involved in phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Elevated expression of SR-BI has been reported in a wide variety of malignant cell lines such as breast, prostate, ovarian, pancreatic, nasopharyngeal, and colorectal cancers. In hepatocytes, it is reported to act as a receptor for hepatitis C virus and facilitates virus entry into cells. SR-B1 is also described as a silica receptor that through an extracellular -helix specifically recognizes amorphous and crystalline silica. However, it does not recognize titanium dioxide nanoparticles, latex nanoparticles, or monosodium urate crystals. It is involved in silica-induced pulmonary inflammation in murine models. Clone AOB-1 is shown to specifically bind to murine SR-BI on Jurkat.EcoR cells and blocks silica binding to these cells in a dose-dependent manner. (Ref.: Tsugita, M., et al. (2017). Cell Rep. 18; 1298-1311; Mooberry, LK., et al. (2016). Front. Pharmacol. 7; 466). Webb, NR., et al. (1998). J. Biol. Chem. 273(24); 15241-15248).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG1 in PBS without azide.
Quality LevelMQ200
Applications
ApplicationAnti-SCARB1, clone AOB-1, Cat. No. MABF3047, is a rat monoclonal antibody that detects SCARB1 and is tested for use in Flow Cytometry, Immunohistochemistry, Inhibition Assay, and Western Blotting.
Key Applications
  • Flow Cytometry
  • Western Blotting
  • Immunohistochemistry
  • Inhibition
Application NotesTested Applications

Inhibition Assay; A representative lot of this antibody partially inhibited IL-1β secretion in silica stimulated bone marrow-derived macrophages (Tsugita, M., et al. (2017). Cell Rep.18(5); 1298-1311).

Flow Cytometry Analysis: A representative lot detected SCARB1 in Flow Cytometry application (Tsugita, M., et al. (2017). Cell Rep. 18(5);1298-1311).

Western Blotting Analysis: A representative lot detected SCARB1 in Western Blotting application (Tsugita, M., et al. (2017). Cell Rep. 18(5); 1298-1311).

Immunohistochemistry Applications: A representative lot detected SCARB1 in Immunohistochemistry applications (Tsugita, M., et al. (2017). Cell Rep.18(5);1298-1311).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenNIH3T3 cells transfected with mouse SCARB1 (SR-B1).
EpitopeUnknown
CloneAOB-1
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRat
SpecificityClone AOB-1 is a rat monoclonal antibody that detects Scavenger receptor class B member 1 (SCARB1).
IsotypeIgG1
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Scarb1
Purification MethodProtein G purified
UniProt Number
Molecular Weight56.75 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in mSR-B1/Jurkat.EcoR cells.

Flow Cytometry Analysis (FC): 1.0 µg of this antibody detected SR-B1 in one million Jurkat.EcoR cells expressing murine SR-B1 (SCRAB1).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3047-100UG 04065268529102