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MABF2782-100UG Anti-SARS-CoV-2 nucleocapsid Antibody, clone 7C5-B5

Anti-SARS-CoV-2 nucleocapsid, clone 7C5-B5, Cat. No. MABF2782, is a mouse monoclonal antibody that detects Nucleoprotein of SARS-CoV-2 and is tested for use in Western Blotting.

MABF2782-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Description
Catalogue NumberMABF2782-100UG
DescriptionAnti-SARS-CoV-2 nucleocapsid Antibody, clone 7C5-B5
Alternate Names
  • SARS-CoV-2 Nucleoprotein
  • Nucleocapsid protein
  • SARS-CoV-2 NC
  • Protein N
Background InformationSARS-CoV-2 Nucleoprotein (UniProt: P0DTC9; also known as N, Nucleocapsid protein, NC, Protein N) is encoded by the N gene (Gene ID: 43740575) in SARS-CoV-2 virus. The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. The N protein packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. The RNA binding region of SARS-CoV-2 is localized to amino acids 41-186 and its dimerization domain is in amino acids 258-361. Lack of cysteine is one of the common features in nucleoprotein from different coronaviruses. The complete N protein of SARS-CoV and COV-2 is a highly basic with positive net charges and high pI (10.11). Its N-terminal region is more basic with positive charge, while the C-terminal region is acidic with negative charge. The middle region of nucleoprotein displays relatively higher hydrophobicity and the two termini display greater hydrophilicity. Nucleoprotein can undergo phosphorylation at multiple serines and threonines and some of these phosphorylation events are essential for RNA binding, oligomerization and localization to nucleoli. Phosphorylation is also required for recruitment of host RNA helicase DDX1 (DEAD-Box Helicase 1) that facilitates template readthrough and enables longer subgenomic mRNA synthesis. Clone 7C5-B5 exhibits reactivity only with SARS-CoV-2 and does not react with other coronaviruses.
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-SARS-CoV-2 nucleocapsid, clone 7C5-B5, Cat. No. MABF2782, is a mouse monoclonal antibody that detects Nucleoprotein of SARS-CoV-2 and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected SARS-CoV-2 nucleocapsid in Whole cell lysates of HEK293T cells expressing the nucleocapsid (NC) proteins of various human coronaviruses (Courtesy of Stefan Schüchner, Ingrid Mudrak, Ingrid Frohner, and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged full-length nucleocapsid protein from Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
EpitopeC-terminal
Clone7C5-B5
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 7C5-B5 is a mouse monoclonal antibody that detects nucleocapsid protein of Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It targets an epitope within the C-terminal domain. It does not cross-react with nucleocapsid of common human Coronaviruses.
IsotypeIgG1κ
Species Reactivity
  • Virus
Species Reactivity NoteSARS-CoV-2
Antibody TypeMonoclonal Antibody
Gene Symbol
  • N
Purification MethodProtein G purified
UniProt Number
Molecular Weight~53 kDa observed; 45.63 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysates from HEK293T cells expressing SARS-CoV-2 Nucleocapsid protein.

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected Nucleocapsid protein in lysates from HEK293T cells expressing SARS-CoV-2 Nucleocapsid protein, but not in lysates from the wild type HEK293T cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size100 μg
Global Trade Item Number
Catalogue Number GTIN
MABF2782-100UG 04065266553796