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MABF3090-25UL Anti-SARS-CoV-2 Spike N501Y Antibody, clone 6D4-E2

Anti-SARS-CoV-2 Spike N501Y, clone 6D4-E2, Cat. No. MABF3090, is a mouse monoclonal antibody that detects SARS-CoV-2 Spike protein with N501Y mutation and is tested for use in Immunoprecipitation and Western Blotting.

MABF3090-25UL
25 µL  
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Overview

Replacement Information
Description
Catalogue NumberMABF3090-25UL
DescriptionAnti-SARS-CoV-2 Spike N501Y Antibody, clone 6D4-E2
Alternate Names
  • Spike glycoprotein Alpha N501Y
  • S glycoprotein Alpha N501Y
Background InformationSpike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. Over the course of the pandemic, SARS-CoV2 has consistently mutated giving rise to various mutants. The Alpha variant of SARS-CoV-2 contains 19 nonsynonymous mutations across its viral genome, including 8 substitutions or deletions in the spike protein. The N501Y mutation in Alpha variant results in enhanced viral replication in human airway epithelial cells and is a critical determinant of enhanced infection of the upper airway and transmission. This mutation resides in the ACE2 interface of the receptor binding domain (RBD). Clone 6D4-E2 specifically detects the SARS-CoV-2 Spike mutation N501Y. (Ref.: Liu, Y., et al. (2022). Nature. 602(7896); 294-299; Cheng, L., et al. (2021). Virol. J. 18; 87).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-SARS-CoV-2 Spike N501Y, clone 6D4-E2, Cat. No. MABF3090, is a mouse monoclonal antibody that detects SARS-CoV-2 Spike protein with N501Y mutation and is tested for use in Immunoprecipitation and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
Application NotesTested applications

Western Blotting Analysis: A representative lot detected SARS-CoV-2 spike N501Y in HEK293T transiently transfected with various SARS-CoV-2 variants (Data courtesy of Stefan Schüchner and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

Immunoprecipitation Analysis: A representative lot detected SARS-CoV-2 spike N501Y in Whole cell lysates of HEK293T cells (untransfected or transiently transfected with SARS-CoV-2 spike WT or B.1.351 variant) (Data courtesy of Stefan Schüchner and Egon Ogris (Max Perutz Labs, Medical University of Vienna, Austria).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenA recombinant fusion protein with a scaffold containing a short spike peptide GFQPTYGVGYQ.
EpitopeN-terminal half
Clone6D4-E2
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 6D4-E2 is a mouse monoclonal antibody that detects the alpha variant of SARS-CoV-2 with N501Y mutation in Spike protein.
IsotypeIgG1κ
Species Reactivity
  • Virus
Species Reactivity NoteSARS-CoV-2
Antibody TypeMonoclonal Antibody
Gene Symbol
  • S
Purification MethodProtein G purified
UniProt Number
Molecular Weight~200 kDa observed; 141.18 kDa calculated for full-length Spike protein. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from HEK293T cells transiently transfected with SARS-CoV-2 spike B.1.351, beta.

Western Blotting Analysis: A 1:500 dilution of this antibody detected SARS-CoV-2 spike protein with N501Y mutation in lysate from HEK293T cells transiently transfected with SARS-CoV-2 spike B.1.351, Beta, but not in wild-type HEK293T cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABF3090-25UL 04065269367727