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MABS1990-25UL Anti-RhoB Antibody, clone 7F7

Anti-RhoB, clone 7F7, Cat. No. MABS1990, is a mouse monoclonal antibody that detects Rho-related GTP-binding protein RhoB in human and has been tested for use in ELISA and Western Blotting.

Anti-RhoB Antibody, clone 7F7 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: RHOB, ARH6, ARHB UniProt Number: P62745
MABS1990-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
Mk, Porcine, R, Ch, H, M, Rb, HtELISA, WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1990-25UL
DescriptionAnti-RhoB Antibody, clone 7F7
Alternate Names
  • Rho-related GTP-binding protein RhoB
  • Rho cDNA clone 6
  • h6
Background InformationRho-related GTP-binding protein RhoB (UniProt: P62745; also known as Rho cDNA clone 6, h6) is encoded by the RHOB (also known as ARH6, ARHB) gene (Gene ID: 388) in human. RhoB is a member of the Ras super-family of small GTP-binding proteins, which is involved in hydrolysis of GTP and is active in GTP-bound form. RhoB is a short-lived Rho GTPase whose expression is inducible by a variety of stimuli including growth factors and stress stimuli. Its levels are up-regulated by DNA damaging agents, such as hydrogen peroxide or ionizing radiation. RhoB plays a negative role in tumorigenesis as its deletion is known to cause tumor formation. It is reported to mediate apoptosis in neoplastically transformed cells following DNA damage. It is not considered to be essential for development, but affects cell adhesion and growth factor signaling in transformed cells. RhoB displays 83% homology with RhoA. However, unlike RhoA, which is cytosolic and translocates to plasma membrane upon activation, RhoB localizes to endosomes/multivesicular bodies. RhoB participates in sorting and degradation of growth factors and cytokine receptors. RhoB has three nucleotide-binding regions (aa 12-19; 59-63; and 117-120). RhoB is shown to be critically required for the inflammatory response of endothelial cells to TNF alpha, possibly through MAP kinase activation downstream of the TNFR.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-RhoB, clone 7F7, Cat. No. MABS1990, is a mouse monoclonal antibody that detects Rho-related GTP-binding protein RhoB in human and has been tested for use in ELISA and Western Blotting.
Key Applications
  • ELISA
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:2,000 dilution from a representative lotdetected RhoB in 10 µg of  MCF7 cell lysate.
Western Blotting Analysis: A representative lot detected RhoB in mouse and rabbit spleen, mouse kidney, recombinant RhoB, 293 cells expressing RhoB, CHO, H9C2, PK1P.185, COS, MDA MB-231, MCF7 and chicken fibroblasts   (Courtesy of   Lisa Laury-Kleintop, Ph.D. , Lankenau Institute for Medical Research, Philadelphia PA, USA).
ELISA Analysis: A representative lot detected RhoB in Plate bound recombinant RhoB   (Courtesy of   Lisa Laury-Kleintop, Ph.D. , Lankenau Institute for Medical Research, Philadelphia PA, USA).
 
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal half of human RhoB.
Clone7F7
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 7F7 detects RhoB in multiple species. It targets an epitope within 19 amino acids from the C-terminal half. This clone specifically detects RhoB, but not RhoA, Rac1, or cdc42.
IsotypeIgG1
Species Reactivity
  • Monkey
  • Porcine
  • Rat
  • Chicken
  • Human
  • Mouse
  • Rabbit
  • Hamster
Species Reactivity NoteChicken, Mouse, Hamster, Human, Monkey, Porcine, Rabbit, Rat
Antibody TypeMonoclonal Antibody
Gene Symbol
  • RHOB
  • ARH6
  • ARHB
Purification MethodProtein G purified
UniProt Number
Molecular Weight~20 kDa observed; 22.12 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected RhoB in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABS1990-25UL 04054839551192