Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

MABC1220-25UG Anti-RGS2 Antibody, clone 9D9.1

Detect RGS2 using this mouse monoclonal Anti-RGS2, clone 9D9.1 Antibody, Cat. No. MABC1220, validated for use in Western Blotting and Immunohistochemistry (Paraffin).

Anti-RGS2 Antibody, clone 9D9.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: GS2, G0S8, GIG31 UniProt Number: P41220
MABC1220-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1220-25UG
DescriptionAnti-RGS2 Antibody, clone 9D9.1
Alternate Names
  • Regulator of G-protein signaling 2
  • Cell growth-inhibiting gene 31 protein
  • G0/G1 switch regulatory protein 8
Background InformationRegulator of G-protein signaling 2 (UniProt: P41220; also known as RGS2, Cell growth-inhibiting gene 31 protein, G0/G1 switch regulatory protein 8) is encoded by the RGS2 (also known as G0S8, GIG31) gene (Gene ID: 5997) in human. RGS2 is shown to regulate G protein-coupled receptor signaling cascades. It inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. It also plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. RGS2 is reported to play a role in regulating the constriction and relaxation of vascular smooth muscle and also has a protective effect against myocardial hypertrophy and atrial arrhythmias. It inhibits beta-adrenergic receptor-induced cardiomyocyte hypertrophy. Overexpression of RGS2 reduces the activity of isoproterenol-induced ERK1/2 and prevents AKT activation. Four isoforms of RGS2 have been reported that are produced by alternative splicing. (Ref.: Nunn, C et al. (2010). Cell Signal 22(8); 1231-1239).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect RGS2 using this mouse monoclonal Anti-RGS2, clone 9D9.1 Antibody, Cat. No. MABC1220, validated for use in Western Blotting and Immunohistochemistry (Paraffin).
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot  detected RGS2 in 10 µg of HL-60 cell lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected RGS2 in human cerebral cortex, human pancreas, human lymphoma, and rat testis tissues.
Biological Information
ImmunogenGST/His-tagged recombinant fragment corresponding to 82 amino acids from the N-terminal region of human Regulator of G-protein signaling 2 (RGS2).
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 9D9.1 specifically detects Regulator of G-protein signaling 2 (RGS2) in human and rat cells. It targets an epitope within 82 amino acids from the N-terminal region.
IsotypeIgG2a
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat..
Antibody TypeMonoclonal Antibody
Gene Symbol
  • GS2
  • G0S8
  • GIG31
Purification MethodProtein G purified
UniProt Number
Molecular Weight25 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected RGS2 in 10 µg of Raji cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC1220-25UG 04054839447983