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MABC966 Anti-Perforin-1 Antibody, clone dG9

Anti-Perforin-1 Antibody, clone dG9 is an antibody against Perforin-1 for use in Flow Cytometry, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry.

Anti-Perforin-1 Antibody, clone dG9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: PRF1, PFP UniProt Number: P14222
MABC966
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, IP, ICC, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC966
DescriptionAnti-Perforin-1 Antibody, clone dG9
Alternate Names
  • Perforin-1
  • P1
  • Cytolysin
  • Lymphocyte pore-forming protein
  • PFP
  • Clone deltaG9
Background InformationPerforin-1 (UniProt P14222; also known as Cytolysin, Lymphocyte pore forming protein, P1, PFP) is encoded by the PRF1 (Also known as FLH2, HPLH2, PFN1, PFP) gene in human (Gene ID 5551). Perforin is a secreted protein synthesized by activated cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. It is a key component of the secreted lytic granule of CTL and NK cells. Perforin inserts into the phospholipid bilayer of the target cell plasma membrane, and polymerises to form a pore of ∼16 nm that causes target cell death by osmotic lysis and by allowing other lytic granule components, most notably granzyme B, to enter the targeted cells. Perforin is synthesized as a 70 kDa inactive precursor which is cleaved at the C-terminus to yield a 60 kDa active form.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-Perforin-1 Antibody, clone dG9 is an antibody against Perforin-1 for use in Flow Cytometry, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry.
Key Applications
  • Flow Cytometry
  • Immunoprecipitation
  • Immunocytochemistry
  • Immunohistochemistry
Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated Perforin-1 in YT cell lysate with or without Endo H treatment (Uellner, R., et al. (1997). EMBO. 16(24):7287-7296).
Immunocytochemistry Analysis: A representative lot detected Perforin-1 in human lymphokine-activated killer (LAK) cells (Hameed, A., et al. (1992). American J. of Pathology. 140(5):1025-1030).
Immunohistochemistry Analysis: A representative lot detected Perforin-1 in human cardiac biopsy samples during allograft rejection (Hameed, A., et al. (1992). American J. of Pathology. 140(5):1025-1030).
Biological Information
ImmunogenPurified granules from the human cytotoxic large granular lymphocytes lymphoma line YT corresponding to human Perforin-1.
ClonedG9
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityReacts with non-denatured human perforin precursor and mature perforin, but not denatured perforin.
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PRF1
  • PFP
Purification MethodProtein G Purified
UniProt Number
Molecular Weight61 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in human peripheral blood mononuclear cells.

Flow Cytometry Analysis: 3.0 µg of this antibody detected Perforin-1 in human peripheral blood mononuclear cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC966 04055977169843