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CB1016 Anti-Paxillin Mouse mAb (M107)

Anti-Paxillin Mouse mAb (M107) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CB1016
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~68 kDa paxillin protein in A431 cells.
Catalogue NumberCB1016
Brand Family Calbiochem®
Application Data
Detection of human paxillin by immunoblotting. Samples: Whole cell lysate from A431 cells left untreated (-) or treated with EGF (+; 100 ng/ml for 5 min). Primary antibodies: Anti-Paxillin Mouse mAb (M107) (1:1000) (panel A) and Anti-Paxillin (pSer178) (panel B). Detection: chemiluminescence.
References
ReferencesHuang, C., et al. 2004. Cell Cycle 3, 4.
Woodrow, M.A., et al. 2003. Exp. Cell Res. 287, 325.
Huang, C., et al. 2003. Nature 424, 219.
Product Information
FormLiquid
FormulationIn PBS, 1 mg/ml BSA, 50% glycerol.
Preservative≤ 0.1% sodium azide
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesELISA (1:2000)
Immunoblotting (1:1000)
Immunocytochemistry (1:500)
Immunoprecipitation (1-5 µl)
Application CommentsFor immunoblotting the antibody should be diluted in PBS, 5% BSA, 0.04% Tween®-20 detergent for 1 h at room temperature. Specificity for total paxillin was confirmed by comparison to a phospho-specific anti-paxillin antibody (see application data). Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenfull-length, recombinant, human paxillin
ImmunogenHuman
CloneM107
HostMouse
IsotypeIgG₁
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Irritant
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
CB1016 0

Documentation

Anti-Paxillin Mouse mAb (M107) Certificates of Analysis

TitleLot Number
CB1016

References

Reference overview
Huang, C., et al. 2004. Cell Cycle 3, 4.
Woodrow, M.A., et al. 2003. Exp. Cell Res. 287, 325.
Huang, C., et al. 2003. Nature 424, 219.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-August-2007 RFH
ApplicationELISA (1:2000)
Immunoblotting (1:1000)
Immunocytochemistry (1:500)
Immunoprecipitation (1-5 µl)
Application Data
Detection of human paxillin by immunoblotting. Samples: Whole cell lysate from A431 cells left untreated (-) or treated with EGF (+; 100 ng/ml for 5 min). Primary antibodies: Anti-Paxillin Mouse mAb (M107) (1:1000) (panel A) and Anti-Paxillin (pSer178) (panel B). Detection: chemiluminescence.
DescriptionProtein A purified mouse monoclonal antibody. Recognizes the ~68 kDa paxillin protein.
BackgroundPaxillin is a focal adhesion protein that is involved in the formation of focal adhesions during cell adhesion and migration. The native protein contains LD motifs, LIM domains, and SH3-/SH2-binding domains that facilitate a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin at Ser178 by JNK1 and cdc2 occurs in response to growth factor activation and fibronectin interaction. Mutation of Ser178 to alanine results in a decrease in the migration of keratinocytes and epithelial cells, suggesting that phosphorylation at this site is an important factor in cell migration.
HostMouse
Immunogen speciesHuman
Immunogenfull-length, recombinant, human paxillin
CloneM107
IsotypeIgG₁
Specieschicken, human, mouse, rat
FormLiquid
FormulationIn PBS, 1 mg/ml BSA, 50% glycerol.
Preservative≤ 0.1% sodium azide
CommentsFor immunoblotting the antibody should be diluted in PBS, 5% BSA, 0.04% Tween®-20 detergent for 1 h at room temperature. Specificity for total paxillin was confirmed by comparison to a phospho-specific anti-paxillin antibody (see application data). Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Irritant
ReferencesHuang, C., et al. 2004. Cell Cycle 3, 4.
Woodrow, M.A., et al. 2003. Exp. Cell Res. 287, 325.
Huang, C., et al. 2003. Nature 424, 219.