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MABE629 Anti-PRKDC, clone 3H6 Antibody

Anti-PRKDC, clone 3H6 Antibody is an antibody against PRKDC for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, ELISA.

Anti-PRKDC, clone 3H6 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: PRKDC, HYRC, HYRC1 UniProt Number: P78527
MABE629
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHC, ICC, ELISAMAscitesMonoclonal Antibody
Description
Catalogue NumberMABE629
Replaces04-1024
DescriptionAnti-PRKDC, clone 3H6 Antibody
Alternate Names
  • DNA-dependent protein kinase catalytic subunit
  • DNA-PK catalytic subunit
  • DNA-PKcs
  • PRKDC
Background InformationDNA-dependent protein kinase catalytic subunit (DNA-PK catalytic subunit or DNA-PKcs), also known as DNPK1 or p460, and encoded by the gene name PRKDC or HYRC, is a nuclear protein kinase that is involved in DNA nonhomologous end joining (NHEJ) required to protect and align broken ends of DNA and V(D)J recombination. DNA-PK/PRKDC must be bound to DNA to express its catalytic properties. Autophosphorylation of DNA-PK/PRKDC correlates with loss of protein kinase activity and dissociation of the DNA-PKcs-Ku complex. DNA-PK/PRKDC phosphorylates Hsp90, p53 and c-Jun.
References
Product Information
FormatAscites
PresentationMouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-PRKDC, clone 3H6 Antibody is an antibody against PRKDC for use in Western Blotting, Immunohistochemistry, Immunocytochemistry, ELISA.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Immunocytochemistry
  • ELISA
Application NotesImmunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected PRKDC in breast cancer, colon cancer, lung cancer, and brain tissues.

Immunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected PRKDC in HeLa cells.

ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Biological Information
ImmunogenPurified recombinant fragment of human PRKDC expressed in E. Coli.
Clone3H6
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • PRKDC
  • HYRC
  • HYRC1
Purification MethodUnpurified
UniProt Number
Molecular Weight~47 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 and PRKDC -- hIgGFc transfected HEK293 cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected PRKDC in HEK293 and hIgGFc transfected HEK293 cell lysates.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE629 04055977171730