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MABE1907-25UG Anti-POT1 Antibody, clone 43B7

Anti-POT1, clone 43B7, Cat. No. MABE1907, is a mouse monoclonal antibody that detects POT1 and is tested for use in Chromatin Immunoprecipitation, Immunofluorescence, Immunoprecipitation, Western Blotting, and Western Blotting using KO lysates.

MABE1907-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABE1907-25UG
DescriptionAnti-POT1 Antibody, clone 43B7
Alternate Names
  • Protection of telomeres protein 1
  • POT1-like telomere end-binding protein
Background InformationProtection of telomeres protein 1 (UniProt: Q9NUX5; also known as hPot1, POT1-like telomere end-binding protein) is encoded by the POT1 gene (Gene ID: 25913) in human. POT1 is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. As a component of the shelterin complex, it is involved in the regulation of telomere length and protection. It also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. It binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Its DNA binding domains are localized to amino acids 33-48 and 270-273. POT1 contains two copies of oligonucleotide oligosaccharide-binding fold (OB-fold) domains in its N-terminal half that are responsible for single strand DNA binding activity. TPP1, a shelterin component, plays critical role in chromosome end protection and telomere length regulation and POT1 is shown to negatively regulate TPP1 phosphorylation at serine Ser255 by NIMA Related Kinase 6 (NEK6). (Ref.: Hirai, Y., et al. (2016). Genes Cells 21(8); 874-889; Hirai, Y., et al. (2012). Genes Cells. 17(3); 186-204).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-POT1, clone 43B7, Cat. No. MABE1907, is a mouse monoclonal antibody that detects POT1 and is tested for use in Chromatin Immunoprecipitation, Immunofluorescence, Immunoprecipitation, Western Blotting, and Western Blotting using KO lysates.
Key Applications
  • Western Blotting
  • Chromatin Immunoprecipitation (ChIP)
  • Immunofluorescence
  • Western Blot Using KO Lysates
  • Immunoprecipitation
Application NotesTested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected POT1 in HEK293T, HepG2, and K562 cell lysates.

Immunofluorescence Analysis: A representative lot detected POT1 in Immunofluorescence application. (Hirai, Y., et al. (2012). Genes Cells. 17(3):186-204).

Immunoprecipitation Analysis: A representative lot immunoprecipitated POT1 in Immunoprecipitation application. (Hirai, Y., et al. (2012). Genes Cells. 17(3):186-204; Hirai, Y. et al. (2016). Genes Cells. (8): 874-889).
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected POT1 in Chromatin Immunoprecipitation applications (Hirai, Y., et al. (2012). Genes Cells. 17(3):186-204).

Western Blotting using knockout lysates: A representative lot detected POT1 in wild-type, but not in KO lysates in Western Blotting application. (Hirai, Y., et al. (2012). Genes Cells. 17(3):186-204).

Western Blotting Analysis: A representative lot detected POT1 in Western Blotting application. (Hirai, Y. et al. (2016). Genes Cells. (8): 874-889).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenFull-length recombinant human Protection of telomeres protein 1 (POT1).
EpitopeN-terminal
Clone43B7
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 43B7 is a mouse monoclonal antibody that detects human Protection of telomeres protein 1 (POT1).
IsotypeIgG2aκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • POT1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~70 kDa observed; 71.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Protection of telomeres protein 1 (POT1) in HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABE1907-25UG 04065266876376