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MABC942 Anti-PLA2R Antibody, clone 5F5.1

This Anti-PLA2R Antibody, clone 5F5.1 is validated for use in Western Blotting and Immunohistochemistry for the detection of PLA2R.

Anti-PLA2R Antibody, clone 5F5.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: PLA2R1, CLEC13C UniProt Number: Q13018
MABC942
100 μg  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IHCMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC942
DescriptionAnti-PLA2R Antibody, clone 5F5.1
Alternate Names
  • PLA2-R
  • PLA2R
  • Secretory phospholipase A2 receptor
  • 180 kDa secretory phospholipase A2 receptor
  • C-type lectin domain family 13 member C
  • M-type receptor
Background InformationSecretory phospholipase A2 receptor (UniProt Q13018; also known as 180 kDa secretory phospholipase A2 receptor, C-type lectin domain family 13 member C, M-type receptor, Phospholipase A2 receptor 1) is encoded by the PLA2R1 (also known as CLEC13C, PLA2G1R, PLA2R, PLA2IR) gene (Gene ID 22925) in human. PLA2R is initially produced with a signal peptide sequence (a.a. 1-20), the cleavage of which yields the mature type I transmembrane receptor composed of a short cytoplasmic tail (a.a. 1419-1463), a transmembrane segment (a.a. 1419-1463), and a large extracellular portion consisting of an N-terminal cystein-rich region, a fibronectin-like type II domain, and a tandem repeat of eight carbohydrate-recognition domains (CRDs). Both group IB and X secretory phospholipase A2 (sPLA2) enzymes are identified as ligands orf PLA2R, while group IIA sPLA2s bind PLA2R with about 10-fold lower affinity when compared with group IB and X sPLA2s. In mice, the extracellular portion of PLA2R is reported to be cleaved off into a soluble form (sPLA2R) in blood, where it functions as a circulating sPLA2 inhibitory protein (PLI) that blocks both the receptor-binding and enzymatic activity of sPLA2.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-PLA2R Antibody, clone 5F5.1 is validated for use in Western Blotting and Immunohistochemistry for the detection of PLA2R.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected PLA2R in 10 µg of human kidney tissue lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected PLA2R in human kidney tissue.
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the extracellular C-type lectin domains 3-5 of human PLA2R.
EpitopeExtracellular C-type lectin domains 3-5
Clone5F5.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityExpected to react with both spliced isoforms, as well as the soluble receptor (sPLA2R) derived from metalloproteinases-mediated cleavage of the membrane-bound receptor.
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PLA2R1
  • CLEC13C
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~180 kDa observed. Target band(s) appears larger than the calculated molecular weights (166.6 kDa/isoform 1 and 150.8 kDa/isoform 2) due to posttranslational glycosylation. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human lung tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected PLA2R in 10 µg of human lung tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABC942 04055977296426

Documentation

Anti-PLA2R Antibody, clone 5F5.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-PLA2R Antibody, clone 5F5.1 Certificates of Analysis

TitleLot Number
Anti-PLA2R, clone 5F5.1 - 2986013 2986013
Anti-PLA2R, clone 5F5.1 - 3245601 3245601
Anti-PLA2R, clone 5F5.1 - 3316667 3316667
Anti-PLA2R, clone 5F5.1 - 3681231 3681231
Anti-PLA2R, clone 5F5.1 - 3856166 3856166
Anti-PLA2R, clone 5F5.1 - 3904663 3904663
Anti-PLA2R, clone 5F5.1 - 4019034 4019034
Anti-PLA2R, clone 5F5.1 - 4093167 4093167
Anti-PLA2R, clone 5F5.1 - 4093169 4093169
Anti-PLA2R, clone 5F5.1 -Q2614585 Q2614585
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