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OP74 Anti-PKC (Ab-2) Mouse mAb (MC5)

This Anti-PKC (Ab-2) Mouse mAb (MC5) is validated for use in Blocking Studies, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of PKC (Ab-2).

Anti-PKC (Ab-2) Mouse mAb (MC5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Protein Kinase C

OP74
  
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, H, M, RMMonoclonal Antibody
Description
Overview

This product has been discontinued.



Recognizes the ~79-80 kDa protein kinase C α/β1/β2 isoforms in NIH3T3 cells and brain tissue.
Catalogue NumberOP74
Brand Family Calbiochem®
SynonymsAnti-Protein Kinase C
References
ReferencesXiao, H., et al. 1994. J. Neurosurg. 81, 734.
Jin, L., et al. 1993. Am. J. Pathol. 142, 569.
Nishikawa, M., and Shirakawa, S., 1992. Leuk. Lymphoma 8, 201.
Wetsel, W.C., et al. 1992. J. Cell. Biol. 117, 121.
Clark, E.A., et al. 1991. Lab. Invest. 64, 35.
Hirabayashi, N., et al. 1990. Mol. Carcinog. 3, 171.
Nishizuka, Y., 1989. Cancer 63, 1892.
Yong, S., et al. 1988. Eur. J. Biochem. 173, 247.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Positive controlNIH3T3 cells or brain tissue
Preservative≤0.1% sodium azide
Applications
Application ReferencesImmunoprecipitation Yong, S., et al. 1988. Eur. J. Biochem. 173, 247. Original Clone Yong, S., et al. 1988. Eur. J. Biochem. 173, 247.
Key Applications Immunoblotting (Western Blotting)
Immunoprecipitation
Neutralization Studies
Paraffin Sections
Application NotesBlocking Studies (5 µg/ml, see comments)
Immunoblotting (1 µg/ml)
Immunoprecipitation (see application references)
Paraffin Sections (5 µg/ml, no pre-treatment required)
Application CommentsMay be used to block the down regulation of PKC activity. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenpurified bovine brain PKC
ImmunogenBovine
Epitopewithin the hinge region of PKC
CloneMC5
HostMouse
IsotypeIgG2a
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
OP74 0

Documentation

Anti-PKC (Ab-2) Mouse mAb (MC5) SDS

Title

Safety Data Sheet (SDS) 

Anti-PKC (Ab-2) Mouse mAb (MC5) Certificates of Analysis

TitleLot Number
OP74

References

Reference overview
Xiao, H., et al. 1994. J. Neurosurg. 81, 734.
Jin, L., et al. 1993. Am. J. Pathol. 142, 569.
Nishikawa, M., and Shirakawa, S., 1992. Leuk. Lymphoma 8, 201.
Wetsel, W.C., et al. 1992. J. Cell. Biol. 117, 121.
Clark, E.A., et al. 1991. Lab. Invest. 64, 35.
Hirabayashi, N., et al. 1990. Mol. Carcinog. 3, 171.
Nishizuka, Y., 1989. Cancer 63, 1892.
Yong, S., et al. 1988. Eur. J. Biochem. 173, 247.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-October-2007 RFH
SynonymsAnti-Protein Kinase C
ApplicationBlocking Studies (5 µg/ml, see comments)
Immunoblotting (1 µg/ml)
Immunoprecipitation (see application references)
Paraffin Sections (5 µg/ml, no pre-treatment required)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with X-63 mouse myeloma cells (see application references). Recognizes ~79-80 kDa α, β1, and β2 isoforms of protein kinase C.
BackgroundThe protein kinase C (PKC) family of protein consists of 12 known isozyme members which are classified as Ca++/phosphatidylserine-dependent, serine/threonine kinases. In addition to their involvement in signal transduction they are also involved with cell proliferation, differentiation and function. PKCγ is found primarily in the brain and spinal cord. Expression in other tissues including epidermal, adrenal, haematopoietic, and some tumors is low or variable.
HostMouse
Immunogen speciesBovine
Immunogenpurified bovine brain PKC
Epitopewithin the hinge region of PKC
CloneMC5
IsotypeIgG2a
Speciesbovine, human, mouse, rat
Positive controlNIH3T3 cells or brain tissue
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsMay be used to block the down regulation of PKC activity. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesXiao, H., et al. 1994. J. Neurosurg. 81, 734.
Jin, L., et al. 1993. Am. J. Pathol. 142, 569.
Nishikawa, M., and Shirakawa, S., 1992. Leuk. Lymphoma 8, 201.
Wetsel, W.C., et al. 1992. J. Cell. Biol. 117, 121.
Clark, E.A., et al. 1991. Lab. Invest. 64, 35.
Hirabayashi, N., et al. 1990. Mol. Carcinog. 3, 171.
Nishizuka, Y., 1989. Cancer 63, 1892.
Yong, S., et al. 1988. Eur. J. Biochem. 173, 247.
Application referencesImmunoprecipitation Yong, S., et al. 1988. Eur. J. Biochem. 173, 247. Original Clone Yong, S., et al. 1988. Eur. J. Biochem. 173, 247.