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MABN1602 Anti-PIT-1 (POU1F1) Antibody, clone 8B6.1

Anti-PIT-1 (POU1F1), clone 8B6.1 Antibody, Cat. No. MABN1602, is a highly specific mouse monoclonal antibody, that targets POU1F1 and has been tested in Western Blotting.

Anti-PIT-1 (POU1F1) Antibody, clone 8B6.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: POU1F1, CPHD1, GHF1, PIT1 UniProt Number: P28069
MABN1602
100 µL  
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Key Spec Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABN1602
DescriptionAnti-PIT-1 (POU1F1) Antibody, clone 8B6.1
Alternate Names
  • Pituitary-specific positive transcription factor 1
  • GHF-1
  • Growth hormone factor 1
  • PIT-1
Background InformationPituitary-specific positive transcription factor 1 (UniProt P28069; also known as GHF-1, Growth hormone factor 1, PIT-1) is encoded by the POU1F1 (also known as CPHD1, GHF1, PIT1) gene (Gene ID 5449) in human. PIT-1 is a transcription factor that serves as a master regulator of lineage commitment and expansion during the anterior pituitary development process that drives regional expansion and functional differentiation of five distinct hormone-producing cell lineages, including somatotropes, lactotropes, thyrotropes, corticotropes, and gonadotropes. Loss of Pit-1 expression results in a combined pituitary hormone deficiency (CPHD) syndrome reflecting the absence of lactotropes, somatotropes, and a subset of thyrotropes. Although originally characterized as pituitary-specific, PIT-1 is also expressed in human breast. PIT-1 upregulation in breast tumors increases cell proliferation and the expression of two breast cancer-related hormones, growth hormone (GH) and prolactin (PRL), leading to MMPs upregulation and breast cancer metastasis. Human POU1F1 gene encodes two alternatively spliced isoforms, where Pit-1A (GHF-2) a.a. 47-73 is truncated to a single Thr47 residue in Pit-1B (GHF-1). Both PIT-1 isoforms contain a PIT-1 POU-specific domain (a.a. 150-224 of Pit-1A or a.a. 124-198 of Pit-1B) and a DNA-binding Homeobox (a.a. 240-299 of Pit-1A or a.a. 214-273 of Pit-1B).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PIT-1 (POU1F1), clone 8B6.1 Antibody, Cat. No. MABN1602, is a highly specific mouse monoclonal antibody, that targets POU1F1 and has been tested in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant human PIT-1 internal fragment.
Clone8B6.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 8B6.1 epitope lies within an internal region present in both human PIT-1 spliced isoform A (Pit-1A, GHF-2) and isoform B (Pit-1B, GHF-1).
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • POU1F1
  • CPHD1
  • GHF1
  • PIT1
Purification MethodProtein G purified.
UniProt Number
Molecular Weight~33 kDa observed. 35.67 kDa (isoform A, Pit-1A, GHF-2) and 32.91 kDa (isoform B, Pit-1B, GHF-1) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human pituitary tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PIT-1/POU1F1 in 10 µg of human pituitary tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MABN1602 04054839055065