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ST1647 Anti-PGK1 Mouse mAb (2H4)

Anti-PGK1 Mouse mAb (2H4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Phosphoglycerate Kinase 1

ST1647
  
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Overview

Replacement Information

Key Spec Table

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~37 kDa PGK1 protein in HepG2 cells.
Catalogue NumberST1647
Brand Family Calbiochem®
SynonymsAnti-Phosphoglycerate Kinase 1
Application Data
Detection of human PGK1 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-PGK1 Mouse mAb (2H4) (Cat. No. ST1647) (1-5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHepG2 cells
PreservativeNone
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponing to amino acids of 321-418 human PGK1, expressed as a GST fusion protein
Clone2H4
HostMouse
IsotypeIgG2a
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
ST1647 0

Documentation

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-November-2010 RFH
SynonymsAnti-Phosphoglycerate Kinase 1
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Application Data
Detection of human PGK1 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-PGK1 Mouse mAb (2H4) (Cat. No. ST1647) (1-5 µg/ml). Detection: chemiluminescence.
DescriptionPurified mouse monoclonal antibody. Recognizes the ~37 kDa PGK1 protein.
BackgroundThe protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. This gene lies on the X-chromosome, while a related pseudogene also has been found on the X-chromosome and another on chromosome 19.
HostMouse
ImmunogenA recombinant polypeptide corresponing to amino acids of 321-418 human PGK1, expressed as a GST fusion protein
Clone2H4
IsotypeIgG2a
Specieshuman
Positive controlHepG2 cells
FormLiquid
FormulationIn PBS, pH 7.2.
PreservativeNone
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review