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ST1202 Anti-PGC-1α Mouse mAb (4C1.3)

This Anti-PGC-1α Mouse mAb (4C1.3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation, Paraffin Sections for the detection of PGC-1α.

Anti-PGC-1α Mouse mAb (4C1.3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Peroxisome Proliferator-Activated Receptor- γ Coactivator 1 α

ST1202
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Products

Catalogue NumberPackaging Qty/Pack
ST1202-1SET Plastic ampoule 1 set
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Description
OverviewRecognizes the endogenous forms of PGC-1α, which includes the ~113 kDa PGC-1α protein and the ~38 kDa splice variant, in brown adipose tissue, liver, and kidney.
Catalogue NumberST1202
Brand Family Calbiochem®
SynonymsAnti-Peroxisome Proliferator-Activated Receptor- γ Coactivator 1 α
Application Data
Detection of human PGC-1α by immunoblottiong. Samples: Whole cell lysates from COS cells transformed with wild-type PGC-1α (5 µg, lane 1; 10 µg, lane 2; 20 µg, lane 3), whole cell extract from brown adipose tissue of a cold-exposed mouse (lane 4), and whole cell lysate from CHO cells transformed with NT-PGC-1α (0.5 µg, lane 5). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection chemiluminescence.

Detection of mouse PGC-1α by immunoblottiong. Samples: NT-PGC-1α Positive Control, (lane 1), whole cell extract from brown adipose tissue of mice kept at room temperature (50 µg; lanes 2-4), whole cell extract from brown adipose tissue of mice exposed to 4°C for 5 h (50 µg; lanes 5-7), and PGC-1α positive control (lane 8). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection: chemiluminescence.

Detection of mouse PGC-1α by immunoblottiong. Samples: PGC-1α positive control (lane 1), nuclear extract from mouse liver (75 µg, lane 2), whole cell extract from mouse kidney (100 µg, lane 3), and whole cell extract from kidney of a PGC-1α knock-out mouse (100 µg, lane 4). Primary antibody: Anti-PGC-1α Mouse mAb (4C1.3) (Cat. No. ST1202) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesLai, L., et al. 2008. Genes Dev. 14, 1948.
Rodgers, J.T., et al. 2008. FEBS Lett. 582, 46.
Mazzucotelli, A., et al. 2007. Diabetes 10, 2467.
Nemoto, S., et al. 2005. J. Biol. Chem. 16, 16456.
Product Information
FormLiquid
FormulationOne vial of 100 µg antibody in 50 mM PBS, see vial for lot-specific concentration. One vial of NT-PGC-1α Positive Control, supplied as 25 µl whole cell extract in RIPA buffer containing 10 mM &beta-mercaptoethanol and 1% SDS; load 10 µl per lane; add sample buffer prior to SDS-PAGE loading.
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesParaffin Sections
Koh, Y.J., et al. 2009. Exp. Mol. Med. 41, 880.
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (1 µg/ml)
Immunocytochemistry (5-10 µg/ml)
Immunoprecipitation (10 µg/ml)
Paraffin Sections (whole mount) (see application references)
Application CommentsEndogenous PGC-1α is found primarily in the nucleus and NT-PGC-1α is found in both cytosolic and nuclear fractions. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of amino acids 1-120 of mouse PGC-1α
ImmunogenMouse
Clone4C1.3
HostMouse
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Safety Information
R PhraseR: 21/22-36/37/38

Harmful in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36/37-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing and gloves.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Global Trade Item Number
Catalogue Number GTIN
ST1202-1SET 04055977224085